Radkowski Marek, Opoka-Kegler Jolanta, Cortes Kamila Caraballo, Bukowska-Ośko Iwona, Perlejewski Karol, Pawełczyk Agnieszka, Laskus Tomasz
Department of Immunopathology, Warsaw Medical University, Warsaw, Poland.
Municipal Hospital for Infectious Diseases, Warsaw, Poland.
J Gen Virol. 2014 Sep;95(Pt 9):2004-2009. doi: 10.1099/vir.0.064709-0. Epub 2014 Jun 11.
Low-level hepatitis C virus (HCV) RNA may persist in PBMCs after successful treatment of chronic hepatitis C, but the consequences of this phenomenon are unclear. Forty-nine patients who achieved a sustained virological response (SVR) after pegylated IFN and ribavirin therapy were analysed 52-66 months after the SVR. HCV RNA was detected in PBMCs from 18 patients (47.4 %), and PBMCs in two patients stained positive for non-structural protein 3 (NS3). Quantification of various cytokine and chemokine transcripts in PBMCs revealed that levels of IL-6, IL-8, IL-12, TNF-α and macrophage inflammatory protein 1β were significantly higher in HCV-positive patients than in HCV-negative individuals. In conclusion, persistence of HCV RNA in PBMCs of patients with a SVR appears to be associated with immune activation.
慢性丙型肝炎成功治疗后，外周血单个核细胞（PBMCs）中可能会持续存在低水平的丙型肝炎病毒（HCV）RNA，但这种现象的后果尚不清楚。对49例在聚乙二醇化干扰素和利巴韦林治疗后获得持续病毒学应答（SVR）的患者在SVR后52 - 66个月进行了分析。在18例患者（47.4%）的PBMCs中检测到HCV RNA，2例患者的PBMCs中非结构蛋白3（NS3）染色呈阳性。对PBMCs中各种细胞因子和趋化因子转录物的定量分析显示，HCV阳性患者中白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-12（IL-12）、肿瘤坏死因子-α（TNF-α）和巨噬细胞炎性蛋白1β（MIP-1β）的水平显著高于HCV阴性个体。总之，SVR患者PBMCs中HCV RNA的持续存在似乎与免疫激活有关。
