Aktories K, Braun U, Rösener S, Just I, Hall A
Rudolf-Buchheim-Institut für Pharmakologie, Giessen, FRG.
Biochem Biophys Res Commun. 1989 Jan 16;158(1):209-13. doi: 10.1016/s0006-291x(89)80199-8.
The ras-related rho A protein expressed in E. coli, was ADP-ribosylated by botulinum ADP-ribosyltransferase C3. C3 also modified the valine-14 mutant rho protein but not the products of H-ras, R-ras, ral, ypt, and rap 1 genes. A ras-rho chimaera consisting of 60 amino acids from the amino terminus of ras fused to 133 amino acids from the carboxy terminus of rho was not modified by C3. Antibodies raised against the porcine brain cytosolic substrate of C3 cross reacted with the rho, valine-14 rho and ras-rho proteins, but not with the gene products of H-ras, R-ras, ral or rap 1. Polyclonal anti-H-ras antibodies cross reacted with H-ras but not with ral, rho, or the C3 substrate purified from porcine brain.
在大肠杆菌中表达的与ras相关的rho A蛋白，被肉毒杆菌ADP-核糖基转移酶C3进行了ADP-核糖基化修饰。C3也修饰了缬氨酸-14突变型rho蛋白，但不修饰H-ras、R-ras、ral、ypt和rap 1基因的产物。由来自ras氨基末端的60个氨基酸与来自rho羧基末端的133个氨基酸融合而成的ras-rho嵌合体未被C3修饰。针对C3的猪脑胞质底物产生的抗体与rho、缬氨酸-14 rho和ras-rho蛋白发生交叉反应，但不与H-ras、R-ras、ral或rap 1的基因产物发生反应。多克隆抗H-ras抗体与H-ras发生交叉反应，但不与ral、rho或从猪脑纯化的C3底物发生反应。
