Pisani Marco, Zucco Massimo
Appl Opt. 2014 May 10;53(14):2983-7. doi: 10.1364/AO.53.002983.
We demonstrate the ability of our hyperspectral imaging device, based on a scanning Fabry-Perot interferometer, to obtain a single hyper-image of a sample marked with different fluorescent molecules, and to unambiguously discriminate them by observing their spectral fingerprints. An experiment carried out with cyanines, fluorescein, and quantum dots emitting in the yellow-orange region, demonstrates the feasibility of multi-labeled fluorescence microscopy without the use of multiple filter sets or dispersive means.
我们展示了基于扫描法布里-珀罗干涉仪的高光谱成像设备的能力，即获取标记有不同荧光分子的样本的单个高光谱图像，并通过观察其光谱指纹来明确区分它们。用在黄橙色区域发射的花青、荧光素和量子点进行的实验，证明了无需使用多个滤光片组或色散手段的多标记荧光显微镜的可行性。
