Thin Film Technology Center/Department of Optics and Photonics, National Central University, Taoyuan 32001, Taiwan.
BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
Sci Rep. 2017 Mar 10;7:44555. doi: 10.1038/srep44555.
White-light scanning interferometry (WLSI) is often used to study the surface profiles and properties of thin films because the strength of the technique lies in its ability to provide fast and high resolution measurements. An innovative attempt is made in this paper to apply WLSI as a time-domain spectroscopic system for localized surface plasmon resonance (LSPR) sensing. A WLSI-based spectrometer is constructed with a breadboard of WLSI in combination with a spectral centroid algorithm for noise reduction and performance improvement. Experimentally, the WLSI-based spectrometer exhibits a limit of detection (LOD) of 1.2 × 10 refractive index units (RIU), which is better than that obtained with a conventional UV-Vis spectrometer, by resolving the LSPR peak shift. Finally, the bio-applicability of the proposed spectrometer was investigated using the rs242557 tau gene, an Alzheimer's and Parkinson's disease biomarker. The LOD was calculated as 15 pM. These results demonstrate that the proposed WLSI-based spectrometer could become a sensitive time-domain spectroscopic biosensing platform.
白光扫描干涉测量法（WLSI）常用于研究薄膜的表面轮廓和特性，因为该技术的优势在于能够提供快速且高分辨率的测量。本文创新性地尝试将 WLSI 用作局域表面等离子体共振（LSPR）传感的时域光谱系统。通过将 WLSI 与光谱质心法相结合，构建了基于 WLSI 的光谱仪，以实现降噪和性能提升。实验中，基于 WLSI 的光谱仪通过分辨 LSPR 峰位移，展现出优于传统紫外可见光谱仪的 1.2×10 折射率单位（RIU）的检测极限（LOD）。最后，使用阿尔茨海默病和帕金森病生物标志物 rs242557 tau 基因，对所提出的光谱仪的生物适用性进行了研究。LOD 计算值为 15 pM。这些结果表明，所提出的基于 WLSI 的光谱仪有望成为一种灵敏的时域光谱生物传感平台。
