Qin Li-Li, Li Meng, Sun Rong, Wu Zhi-Jin, He Kun, Mo Mei-Lan, Wei Tian-Chao, Wei Ping
Bing Du Xue Bao. 2014 Mar;30(2):162-70.
In order to investigate the prevalence and track genetic and antigenic evolutions of infectious bronchitis virus (IBV) and their prevalence in Guangxi, China since 1985, gene amplification and sequencing and virus neutralization (VN) test on chicken embryo tracheal organ cultures were used in genotyping and serotyping of 28 IBV isolates during 2009-2011 in Guangxi. The results of N gene sequencing and comparison showed that the 28 isolates and reference strains were classified into three groups, and most isolates belonged to group Ill, while the isolates in 1985-2008 belonged to groups IV and II. The data of VN test indicated that the 28 isolates belonged to 6 serotypes; among them, 71. 4% belonged to serotypes 1, 2, and 3, and 11 (39.3%) shared the same serotype with the current vaccine strains. Given the data of our previous study, it is found that prevalent serotypes and their proportions varied in different areas of Guangxi and during different periods. These data lay a good foundation for developing an oil-emulsified inactivated polyvalent vaccine containing local dominant serotypes for the effective prevention and control of infectious bronchitis.
为了调查传染性支气管炎病毒（IBV）的流行情况，追踪其基因和抗原演变以及自1985年以来在中国广西的流行情况，对2009 - 2011年广西的28株IBV分离株进行基因扩增、测序以及鸡胚气管器官培养的病毒中和（VN）试验，以进行基因分型和血清分型。N基因测序及比较结果显示，28株分离株和参考毒株被分为三组，大多数分离株属于第III组，而1985 - 2008年的分离株属于第IV组和第II组。VN试验数据表明，28株分离株属于6种血清型；其中，71.4%属于血清型1、2和3，11株（39.3%）与当前疫苗株具有相同血清型。结合我们之前的研究数据发现，广西不同地区以及不同时期流行的血清型及其比例有所不同。这些数据为开发含当地优势血清型的油乳剂灭活多价疫苗以有效预防和控制传染性支气管炎奠定了良好基础。
