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携带禽冠状病毒传染性支气管炎病毒不同结构蛋白组合的三种病毒样颗粒的构建及免疫原性比较

Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious Bronchitis Virus.

作者信息

Zhang Yu, Yuan Yuan, Zhang Li-Hua, Zhu Dan, Wang Lu, Wei Lan-Ping, Fan Wen-Sheng, Zhao Chang-Run, Su Yan-Jing, Liao Jian-Qi, Yong Lu, Wei Tian-Chao, Wei Ping, Mo Mei-Lan

机构信息

College of Animal Science and Technology, Guangxi University, Nanning 530004, China.

出版信息

Vaccines (Basel). 2021 Feb 11;9(2):146. doi: 10.3390/vaccines9020146.

DOI:10.3390/vaccines9020146
PMID:33670249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7918244/
Abstract

Infectious bronchitis virus (IBV) poses massive economic losses in the global poultry industry. Here, we firstly report the construction and immunogenicity comparison of virus-like particles (VLPs) carrying the S, M and E proteins (SME-VLPs); VLPs carrying the S and M proteins (SM-VLPs); and VLPs carrying the M and E proteins (ME-VLPs) from the dominant serotype representative strain GX-YL5 in China. The neutralizing antibody response induced by the SME-VLPs was similar to that induced by the inactivated oil vaccine (OEV) of GX-YL5, and higher than those induced by the SM-VLPs, ME-VLPs and commercial live vaccine H120. More importantly, the SME-VLPs elicited higher percentages of CD4 and CD8 T lymphocytes than the SM-VLPs, ME-VLPs and OEV of GX-YL5. Compared with the OEV of GX-YL5, higher levels of IL-4 and IFN-γ were also induced by the SME-VLPs. Moreover, the mucosal immune response (sIgA) induced by the SME-VLPs in the tear and oral swabs was comparable to that induced by the H120 vaccine and higher than that induced by the OEV of GX-YL5. In the challenge experiment, the SME-VLPs resulted in significantly lower viral RNA levels in the trachea and higher protection scores than the OEV of GX-YL5 and H120 vaccines, and induced comparable viral RNA levels in the kidneys, and tear and oral swabs to the OEV of GX-YL5. In summary, among the three VLPs, the SME-VLPs carrying the S, M and E proteins of IBV could stimulate the strongest humoral, cellular and mucosal immune responses and provide effective protection, indicating that it would be an attractive vaccine candidate for IB.

摘要

传染性支气管炎病毒（IBV）给全球家禽业造成了巨大的经济损失。在此，我们首次报道了携带S、M和E蛋白的病毒样颗粒（SME-VLPs）、携带S和M蛋白的病毒样颗粒（SM-VLPs）以及携带M和E蛋白的病毒样颗粒（ME-VLPs）的构建及其免疫原性比较，这些病毒样颗粒来自中国优势血清型代表性毒株GX-YL5。SME-VLPs诱导的中和抗体反应与GX-YL5灭活油乳剂疫苗（OEV）诱导的相似，且高于SM-VLPs、ME-VLPs和商业活疫苗H120诱导的中和抗体反应。更重要的是，SME-VLPs诱导的CD4和CD8 T淋巴细胞百分比高于SM-VLPs、ME-VLPs以及GX-YL5的OEV。与GX-YL5的OEV相比，SME-VLPs还诱导产生了更高水平的IL-4和IFN-γ。此外，SME-VLPs在泪液和口腔拭子中诱导的黏膜免疫反应（sIgA）与H120疫苗诱导的相当，且高于GX-YL5的OEV诱导的。在攻毒实验中，SME-VLPs导致气管中的病毒RNA水平显著低于GX-YL5的OEV和H120疫苗，保护评分更高，并且在肾脏、泪液和口腔拭子中诱导的病毒RNA水平与GX-YL5的OEV相当。总之，在这三种病毒样颗粒中，携带IBV S、M和E蛋白的SME-VLPs能刺激最强的体液、细胞和黏膜免疫反应并提供有效保护，表明它将是一种有吸引力的IB疫苗候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/bed8655a575d/vaccines-09-00146-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/02f37d26fe02/vaccines-09-00146-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/a6bdf56c10bc/vaccines-09-00146-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/f2289312ae26/vaccines-09-00146-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/953af67a3801/vaccines-09-00146-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/bed8655a575d/vaccines-09-00146-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/02f37d26fe02/vaccines-09-00146-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/a6bdf56c10bc/vaccines-09-00146-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/f2289312ae26/vaccines-09-00146-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/953af67a3801/vaccines-09-00146-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fc4/7918244/bed8655a575d/vaccines-09-00146-g013.jpg

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