鸽Ⅰ型副黏病毒（传沙株）攻毒剂量的研究

[Study on challenge dose of pigeon paramyxovirus type 1 (Chuansha strain)].

作者信息

Zhang Jun-Ping, Yang Hui-Ping, Jiang Feng-Ying, Ni Jian-Ping, Li Chun-Hua

出版信息

Bing Du Xue Bao. 2014 Mar;30(2):177-9.

Abstract

In order to determine the challenge dose of pigeon paramyxovirus type 1 (PPMV-1) inactivated vaccine (S-1 strain). The virus titer of PPMV-1 E5 allantoic fluid (Chuansha strain) was determined using SPF chicken embryos in this research. After inoculating 30-day-old and 120-day-old pigeons with low-HI antibody against PPMV-1 (HI antibody < or =2) with different doses of PPMV-1 (Chuansha strain), the clinical symptoms and histopathological lesions of the challenged pigeons were examined. The results showed that the minimal lethal dose (MLD) of PPMV-1 (Chuansha strain) was 102.5 ELD50, so we determined that 10(5.5) ELD50, which was 1000 times the MLD, could be taken as the challenge dose in the vaccine efficacy test for PPMV-1 inactivated vaccine (S-1 strain).

摘要

为确定鸽Ⅰ型副粘病毒（PPMV-1）灭活疫苗（S-1株）的攻毒剂量。本研究采用SPF鸡胚测定PPMV-1 E5尿囊液（传沙株）的病毒滴度。用不同剂量的PPMV-1（传沙株）接种PPMV-1血凝抑制（HI）抗体效价低（HI抗体≤2）的30日龄和120日龄鸽子后，观察攻毒鸽子的临床症状和组织病理学病变。结果表明，PPMV-1（传沙株）的最小致死剂量（MLD）为102.5 ELD50，因此确定10(5.5) ELD50（为MLD的1000倍）可作为PPMV-1灭活疫苗（S-1株）疫苗效力试验的攻毒剂量。