Yang Bo, Yang Fan, Wang Song-Hao, Zhang Yan, Cao Wei-Jun, Yin Hong, Zheng Hai-Xue
Bing Du Xue Bao. 2014 Mar;30(2):213-20.
Reverse-genetic engineering of foot and mouth disease virus (FMDV) can improve the productivity, antigen matching, antigen stability, immune response ability, and biological safety of vaccines, so vaccine candidates with anticipated biological characteristics can be promptly achieved. Negative influence in taming of virulent strains can also be decreased or avoided. Reverse genetics not only make up for deficiencies like limitation of viral nature, low success rate, and time and energy consuming, but also realize more active designing of vaccines. Therefore, reverse genetics is significant in improving integral quality and efficiency of vaccines. In this review, we use FMDV vaccines as an example to summarize improvement in biological characteristics of virulent strains and provide a reference for related researches.
口蹄疫病毒（FMDV）的反向遗传工程可提高疫苗的生产力、抗原匹配性、抗原稳定性、免疫应答能力和生物安全性，从而能够迅速获得具有预期生物学特性的候选疫苗。同时，还可减少或避免强毒株驯化过程中的负面影响。反向遗传学不仅弥补了诸如病毒特性限制、成功率低以及耗时耗力等不足，还实现了更为主动的疫苗设计。因此，反向遗传学对于提高疫苗的整体质量和效率具有重要意义。在本综述中，我们以FMDV疫苗为例，总结强毒株生物学特性的改进情况，为相关研究提供参考。
