源自雪白根霉葡糖淀粉酶的N-连接糖链的一级结构。
Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus.
作者信息
Takegawa K, Kawasaki N, Iwahara S, Yamamoto K, Tochikura T, Mikami B, Morita Y
机构信息
Department of Bioresource Science, Kagawa University, Japan.
出版信息
Biochim Biophys Acta. 1989 Jan 27;990(1):98-100. doi: 10.1016/s0304-4165(89)80018-2.
The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-beta-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNac-ol.
对雪白根霉葡糖淀粉酶（主要成分）的N - 连接糖链的一级结构进行了研究。碳水化合物部分通过黄杆菌属内切β - N - 乙酰氨基葡糖苷酶消化从多肽主链上释放出来。使用荧光吡啶氨基衍生物的外切糖苷酶消化方法、在Bio - Gel P - 4上的凝胶渗透色谱法以及400 - MHz 1H - NMR光谱法进行的研究表明，最丰富的结构是(Man)8 - GlcNac - ol。
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