原甲变形杆菌内切-β-N-乙酰氨基葡萄糖苷酶的转糖基化活性。
Transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae.
作者信息
Takegawa K, Yamaguchi S, Kondo A, Iwamoto H, Nakoshi M, Kato I, Iwahara S
机构信息
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
出版信息
Biochem Int. 1991 Jul;24(5):849-55.
Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae has transglycosylation activity. Treatment of (Man)6(GlcNAc)2Asn with the enzyme in the presence of free N-acetylglucosamine gave a mixture of (Man)6GlcNAc and (Man)6GlcNAc beta 1----4GlcNAc mixture. N-Acetylglucosamine at the reducing end of the latter sugar chain was found by HPLC of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 MHz 1H-NMR spectroscopy.
来自原光节杆菌的内切-β-N-乙酰氨基葡萄糖苷酶具有转糖基化活性。在游离N-乙酰氨基葡萄糖存在的情况下，用该酶处理(Man)6(GlcNAc)2Asn得到(Man)6GlcNAc和(Man)6GlcNAcβ1----4GlcNAc混合物。通过对碳水化合物组成以及还原端残基的吡啶基氨基衍生物的外切糖苷酶消化产物进行HPLC分析，以及通过400 MHz 1H-NMR光谱法，发现了后一种糖链还原端的N-乙酰氨基葡萄糖。
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