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tuf基因的复制:对真细菌系统发育的新见解。

Duplication of the tuf gene: a new insight into the phylogeny of eubacteria.

作者信息

Sela S, Yogev D, Razin S, Bercovier H

机构信息

Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.

出版信息

J Bacteriol. 1989 Jan;171(1):581-4. doi: 10.1128/jb.171.1.581-584.1989.

DOI:10.1128/jb.171.1.581-584.1989
PMID:2492503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC209627/
Abstract

The conservation and duplication of the tuf gene encoding the elongation factor EF-Tu were used to define phylogenetic relationships among eubacteria. When the tufA gene of Escherichia coli was used as a probe in hybridization experiments, duplicate tuf genes were found in gram-negative bacteria from three major phyla: purple bacteria, bacteroides, and cyanobacteria. Only a single copy of tuf was found in gram-positive bacteria, including mycobacteria and mycoplasmas. Gram-positive clostridia were found to carry two copies of tuf.

摘要

编码延伸因子EF-Tu的tuf基因的保守性和复制情况被用于确定真细菌之间的系统发育关系。当在杂交实验中使用大肠杆菌的tufA基因作为探针时,在三个主要门类的革兰氏阴性菌中发现了重复的tuf基因:紫色细菌、拟杆菌和蓝细菌。在革兰氏阳性菌中仅发现了一个tuf拷贝,包括分枝杆菌和支原体。革兰氏阳性梭菌被发现携带两个tuf拷贝。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0921/209627/ec1bd8aa26b6/jbacter00167-0604-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0921/209627/101af34da2f4/jbacter00167-0604-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0921/209627/ec1bd8aa26b6/jbacter00167-0604-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0921/209627/101af34da2f4/jbacter00167-0604-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0921/209627/ec1bd8aa26b6/jbacter00167-0604-b.jpg

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本文引用的文献

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A phylogenetic definition of the major eubacterial taxa.主要真细菌类群的系统发育定义。
Syst Appl Microbiol. 1985;6:143-51. doi: 10.1016/s0723-2020(85)80047-3.
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Regulation of the expression of tufA and tufB, the two genes coding for the elongation factor EF-Tu in Escherichia coli.大肠杆菌中编码延伸因子EF-Tu的两个基因tufA和tufB的表达调控。
FEBS Lett. 1982 Mar 22;139(2):325-30. doi: 10.1016/0014-5793(82)80881-8.
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The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli.大肠杆菌tufB及四个相邻tRNA结构基因的核苷酸序列。
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Expression of duplicate msa genes in the salmonid pathogen Renibacterium salmoninarum.鲑科鱼类病原体鲑鱼肾杆菌中重复msa基因的表达
Appl Environ Microbiol. 2002 Nov;68(11):5480-7. doi: 10.1128/AEM.68.11.5480-5487.2002.
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The unique tuf2 gene from the kirromycin producer Streptomyces ramocissimus encodes a minor and kirromycin-sensitive elongation factor Tu.来自春日霉素产生菌卷曲链霉菌的独特tuf2基因编码一种次要的、对春日霉素敏感的延伸因子Tu。
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Ribosomal protein gene cluster analysis in eubacterium genomics: homology between Sinorhizobium meliloti strain 1021 and Bacillus subtilis.真细菌基因组学中的核糖体蛋白基因簇分析:苜蓿中华根瘤菌1021菌株与枯草芽孢杆菌之间的同源性
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Evidence for horizontal gene transfer in evolution of elongation factor Tu in enterococci.肠球菌中延伸因子Tu进化过程中水平基因转移的证据。
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Gene. 1980 Dec;12(1-2):33-9. doi: 10.1016/0378-1119(80)90013-x.
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The nucleotide sequence of the cloned tufA gene of Escherichia coli.大肠杆菌克隆的tufA基因的核苷酸序列。
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