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Organization of the genes for protein synthesis elongation factors Tu and G in the cyanobacterium Anacystis nidulans.

作者信息

Mickel F S, Spremulli L L

出版信息

J Bacteriol. 1986 Apr;166(1):78-82. doi: 10.1128/jb.166.1.78-82.1986.

DOI:10.1128/jb.166.1.78-82.1986
PMID:3082860
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214559/
Abstract

The genes for protein synthesis elongation factors Tu and G were cloned from the cyanobacterium Anacystis nidulans. The locations of these genes were mapped within the cloned DNA fragment by hybridization with Escherichia coli probes. The organization of the cloned fragment and the DNA flanking it in the A. nidulans chromosome was also determined. The elongation factor Tu and G genes are adjacent to one another and in the same 5'-to-3' orientation. In contrast to other gram-negative bacteria, A. nidulans contains only one gene for elongation factor Tu.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92b2/214559/550cdc431242/jbacter00209-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92b2/214559/48cde0901553/jbacter00209-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92b2/214559/550cdc431242/jbacter00209-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92b2/214559/48cde0901553/jbacter00209-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92b2/214559/550cdc431242/jbacter00209-0088-b.jpg

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1
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2
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Proc Natl Acad Sci U S A. 1982 Apr;79(7):2264-7. doi: 10.1073/pnas.79.7.2264.

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本文引用的文献

1
Plasmid screening at high colony density.高菌落密度下的质粒筛选
Gene. 1980 Jun;10(1):63-7. doi: 10.1016/0378-1119(80)90144-4.
2
Duplication of the tuf gene, which encodes peptide chain elongation factor Tu, is widespread in Gram-negative bacteria.编码肽链延伸因子Tu的tuf基因的复制在革兰氏阴性细菌中广泛存在。
J Bacteriol. 1981 Dec;148(3):1006-11. doi: 10.1128/jb.148.3.1006-1011.1981.
3
The conservation of DNA sequences over very long periods of evolutionary time. Evidence against intergeneric chromosomal transfer as an explanation for the presence of Escherichia coli tuf gene sequences in taxonomically-unrelated prokaryotes.
两种问号钩端螺旋体血清型遗传图谱的比较为双染色体和种内异质性提供了证据。
J Bacteriol. 1993 Sep;175(17):5445-51. doi: 10.1128/jb.175.17.5445-5451.1993.
4
Genes for the ribosomal proteins S12 and S7 and elongation factors EF-G and EF-Tu of the cyanobacterium, Anacystis nidulans: structural homology between 16S rRNA and S7 mRNA.蓝细菌集胞藻6803的核糖体蛋白S12和S7以及延伸因子EF-G和EF-Tu的基因:16S rRNA与S7 mRNA之间的结构同源性
Mol Gen Genet. 1989 Mar;216(1):25-30. doi: 10.1007/BF00332226.
5
Complete nucleotide sequences of seven eubacterial genes coding for the elongation factor Tu: functional, structural and phylogenetic evaluations.编码延伸因子Tu的七个真细菌基因的完整核苷酸序列:功能、结构和系统发育评估。
Arch Microbiol. 1990;153(3):241-7. doi: 10.1007/BF00249075.
DNA序列在非常漫长的进化时间内的保守性。反对将属间染色体转移作为分类学上不相关的原核生物中存在大肠杆菌tuf基因序列的一种解释的证据。
Eur J Biochem. 1981 Nov;120(1):69-77. doi: 10.1111/j.1432-1033.1981.tb05671.x.
4
The elongation factor EF-Tu and its two encoding genes.延伸因子EF-Tu及其两个编码基因。
Prog Nucleic Acid Res Mol Biol. 1983;30:91-126. doi: 10.1016/s0079-6603(08)60684-4.
5
Two tuf genes in the cyanobacterium Spirulina platensis.
J Bacteriol. 1984 Jul;159(1):407-9. doi: 10.1128/jb.159.1.407-409.1984.
6
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒,一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
7
Location of the tufB promoter of E. coli: cotranscription of tufB with four transfer RNA genes.大肠杆菌tufB启动子的位置:tufB与四个转移RNA基因的共转录
Cell. 1981 Jul;25(1):251-8. doi: 10.1016/0092-8674(81)90250-6.
8
Regulation of the synthesis of E. coli elongation factor Tu.大肠杆菌延伸因子Tu合成的调控
Cell. 1981 Jun;24(3):695-706. doi: 10.1016/0092-8674(81)90096-9.
9
Identification of two copies of the gene for the elongation factor EF-Tu in E. coli.在大肠杆菌中鉴定出延伸因子EF-Tu基因的两个拷贝。
Nature. 1975 Oct 9;257(5526):458-62. doi: 10.1038/257458a0.
10
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.一种用于筛选重组质粒DNA的快速碱性提取方法。
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23. doi: 10.1093/nar/7.6.1513.