López-Robles Guadalupe, Montalvo-Corral Maricela, Burgara-Estrella Alexel, Hernández Jesús
Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C., Km 0.6, Carretera a la Victoria, 83000 Hermosillo, Sonora, Mexico.
Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C., Km 0.6, Carretera a la Victoria, 83000 Hermosillo, Sonora, Mexico.
Vet Microbiol. 2014 Aug 6;172(1-2):323-8. doi: 10.1016/j.vetmic.2014.05.017. Epub 2014 May 22.
The aim of this study was to provide an overview of the epidemiological status of swine influenza viruses in pigs from northwestern Mexico in 2008-2009. A serological and molecular survey was conducted in 150 pigs from 15 commercial farms in Sonora, Mexico (northwestern region of Mexico). The serological data showed that 55% of the sera were positive for the H1N1 subtype, 59% for the H3N2 subtype, and 38% for both subtypes. Overall, 16.6% (25/150) of the samples were positive for type A influenza by qRT-PCR. The phylogenetic analysis of the H1 viruses circulating in northwestern Mexico were grouped into cluster α, from five other clusters previously described. The influenza virus H1 circulating in northwestern Mexico showed 97-100% identity at the nucleotide level among them, 89% identity with other North American strains, 88% with strains from central Mexico, and 85% with the pandemic A/H1N1p2009 virus. Meanwhile, a closer relationship with some influenza viruses from North America (97% nucleotide identity) was found for H3 subtype. In conclusion, our results demonstrated a high circulation of strains similar to those observed in the North American linage among commercial farms in northwestern Mexico, involving of a different lineage virus different to the influenza pandemic of 2009.
本研究的目的是概述2008 - 2009年墨西哥西北部猪群中猪流感病毒的流行病学状况。在墨西哥索诺拉州（墨西哥西北部地区）15个商业农场的150头猪中进行了血清学和分子学调查。血清学数据显示，55%的血清对H1N1亚型呈阳性，59%对H3N2亚型呈阳性，38%对两种亚型均呈阳性。总体而言，16.6%（25/150）的样本通过qRT-PCR检测出甲型流感呈阳性。对在墨西哥西北部传播的H1病毒进行的系统发育分析表明，它们被归类到α簇，与之前描述的其他五个簇不同。在墨西哥西北部传播的流感病毒H1在核苷酸水平上彼此间显示出97 - 100%的同源性，与其他北美毒株的同源性为89%，与墨西哥中部毒株的同源性为88%，与大流行的A/H1N1p2009病毒的同源性为85%。同时，发现H3亚型与一些来自北美的流感病毒有更密切的关系（核苷酸同源性为97%）。总之，我们的结果表明，在墨西哥西北部的商业农场中，与在北美谱系中观察到的毒株相似的毒株有很高的传播率，涉及一种与2009年流感大流行不同的谱系病毒。
