Aslan Mutay, Celmeli Gamze, Özcan Filiz, Kupesiz Alphan
Department of Medical Biochemistry, Akdeniz University Medical School, 07070, Antalya, Turkey,
Clin Exp Med. 2015 Aug;15(3):397-403. doi: 10.1007/s10238-014-0293-6. Epub 2014 Jun 13.
The aim of this study was to determine circulating omega-6, omega-3 polyunsaturated fatty acids and prostaglandin E2 (PGE2) levels in steady state sickle cell disease (SCD) patients. Blood was collected from healthy hemoglobin volunteers and steady state homozygous HbSS patients who had not received blood transfusions in the last 3 months. Plasma levels of arachidonic acid (AA, C20:4n-6), dihomo-gamma-linolenic acid (DGLA, C20:3n-6), eicosapentaenoic acid (EPA, C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3) were determined by an optimized multiple reaction monitoring method using ultrafast liquid chromatography coupled with tandem mass spectrometry. PGE2 was measured in serum samples by enzyme immunoassay. Plasma AA and DGLA were significantly increased while EPA and DHA were significantly decreased in SCD plasma compared to control. Serum PGE2 levels, AA/DHA and AA/EPA ratio was significantly higher in SCD patients when compared to control group. The significant increase in PGE2 levels, AA/EPA and AA/DHA ratio confirms the presence of a proinflammatory state in SCD patients.
本研究的目的是测定稳态镰状细胞病（SCD）患者循环中的ω-6、ω-3多不饱和脂肪酸及前列腺素E2（PGE2）水平。采集了健康血红蛋白志愿者以及在过去3个月内未接受输血的稳态纯合HbSS患者的血液。采用超快速液相色谱-串联质谱联用的优化多反应监测方法测定血浆中花生四烯酸（AA，C20:4n-6）、二高-γ-亚麻酸（DGLA，C20:3n-6）、二十碳五烯酸（EPA，C20:5n-3）和二十二碳六烯酸（DHA，C22:6n-3）的水平。通过酶免疫测定法测定血清样本中的PGE2。与对照组相比，SCD患者血浆中的AA和DGLA显著升高，而EPA和DHA显著降低。与对照组相比，SCD患者血清PGE2水平、AA/DHA及AA/EPA比值显著更高。PGE2水平、AA/EPA和AA/DHA比值的显著升高证实了SCD患者存在促炎状态。
