Feng Jixing, Tang Xiaoqian, Zhan Wenbin
Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China.
Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China.
Fish Shellfish Immunol. 2014 Aug;39(2):407-14. doi: 10.1016/j.fsi.2014.05.034. Epub 2014 Jun 9.
To investigate the role of cytoplasmic dynein in white spot syndrome virus (WSSV) infection, the full-length cDNA of cytoplasmic dynein intermediate chain (FcDYNCI) was cloned in Fenneropenaeus chinensis, which consists of 2582 bp and encodes a polypeptide of 660 amino acids. Sequence analysis and multiple sequence alignment displayed that FcDYNCI was a member of cytoplasmic dynein 1 family. The FcDYNCI mRNA was most highly expressed in hemocytes, which was significantly up-regulated post WSSV infection. At 12 h post infection (hpi), confocal microscopic observation showed that WSSV could be co-localized with cytoplasmic dynein in hemocytes. After silencing by specific FcDYNCI dsRNA, the FcDYNCI mRNA level and the protein amount of FcDYNCI in hemocytes both exhibited a significant reduction, and the expression levels of three WSSV genes ie1, wsv477 and vp28 all exhibited the greatest decreases at 24 hpi. These results suggested that cytoplasmic dynein was involved in WSSV infection.
为研究细胞质动力蛋白在对虾白斑综合征病毒（WSSV）感染中的作用，克隆了中国明对虾细胞质动力蛋白中间链（FcDYNCI）的全长cDNA，其长度为2582 bp，编码一个由660个氨基酸组成的多肽。序列分析和多序列比对显示，FcDYNCI是细胞质动力蛋白1家族的成员。FcDYNCI mRNA在血细胞中表达量最高，在WSSV感染后显著上调。感染后12小时（hpi），共聚焦显微镜观察显示，WSSV可与血细胞中的细胞质动力蛋白共定位。用特异性FcDYNCI双链RNA沉默后，血细胞中FcDYNCI mRNA水平和FcDYNCI蛋白量均显著降低，并且在24 hpi时，三种WSSV基因ie1、wsv477和vp28的表达水平均出现最大程度的下降。这些结果表明，细胞质动力蛋白参与了WSSV感染。
