Yamada Ichiro, Hikishima Keigo, Miyasaka Naoyuki, Tokairin Yutaka, Kawano Tatsuyuki, Ito Eisaku, Kobayashi Daisuke, Eishi Yoshinobu, Okano Hideyuki, Shibuya Hitoshi
Department of Diagnostic Radiology and Oncology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
J Magn Reson Imaging. 2014 Sep;40(3):567-76. doi: 10.1002/jmri.24411. Epub 2013 Oct 31.
To demonstrate the feasibility of diffusion-tensor magnetic resonance imaging (MRI) and tractography as a means of evaluating the individual layers of the normal esophageal wall by using esophageal specimens containing carcinoma.
Twelve esophageal specimens each containing a carcinoma that were preserved in formalin were studied with a 7.0-T small-bore MR system equipped with a four-channel phased-array surface coil. Diffusion-tensor MR images were obtained with a field of view of 50-60 × 25-30 mm, matrix of 256 × 128, section thickness of 1 mm, b value of 1000 sec/mm(2) , and motion-probing gradient in seven noncollinear directions. The diffusion-weighted images, apparent diffusion coefficient (ADC) maps, fractional anisotropy (FA) maps, direction-encoded color FA maps, and tractographic images in the 12 esophageal specimens were compared with the histopathologic findings, which served as the gold standard.
The diffusion-weighted images, ADC maps, FA maps, and direction-encoded color FA maps depicted the normal esophageal wall in all 12 specimens (100%) as consisting of eight layers, which clearly corresponded to the tissue layers of the esophageal wall. The ADC, FA, λ1 , λ2 , and λ3 values of each layer of the normal esophageal wall were significantly different from the corresponding values of the adjacent layer or layers of the esophageal wall. Diffusion-tensor tractographic images were able to selectively display the layers of the normal esophageal wall.
By looking at the normal part of esophageal specimens containing carcinoma, we have demonstrated that diffusion-tensor MRI and tractography are capable of depicting the individual tissue layers of the normal esophageal wall.
通过使用含有癌组织的食管标本，证明扩散张量磁共振成像（MRI）和纤维束成像作为评估正常食管壁各层的一种方法的可行性。
对12个保存在福尔马林中、每个都含有癌组织的食管标本，使用配备四通道相控阵表面线圈的7.0-T小口径MR系统进行研究。扩散张量MR图像的采集参数为：视野50 - 60×25 - 30 mm，矩阵256×128，层厚1 mm，b值1000 sec/mm²，以及在七个非共线方向上的运动探测梯度。将12个食管标本的扩散加权图像、表观扩散系数（ADC）图、各向异性分数（FA）图、方向编码彩色FA图和纤维束成像图与作为金标准的组织病理学结果进行比较。
扩散加权图像、ADC图、FA图和方向编码彩色FA图在所有12个标本（100%）中均显示正常食管壁由八层组成，这与食管壁的组织层明显对应。正常食管壁各层的ADC、FA、λ1、λ2和λ3值与食管壁相邻层或多层的相应值有显著差异。扩散张量纤维束成像图能够选择性地显示正常食管壁的各层。
通过观察含有癌组织的食管标本的正常部分，我们证明了扩散张量MRI和纤维束成像能够描绘正常食管壁的各个组织层。
