U87MG和U343MG-A细胞系之间的蛋白质组学分析：寻找胶质瘤侵袭的候选蛋白
Proteomic Analysis between U87MG and U343MG-A Cell Lines: Searching for Candidate Proteins for Glioma Invasion.
作者信息
Pei Jian, Moon Kyung-Sub, Pan SangO, Lee Kyung-Hwa, Ryu Hyang-Hwa, Jung Tae-Young, Kim In-Young, Jang Woo-Yeol, Jung Chae-Hun, Jung Shin
机构信息
Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea. ; Department of Neurosurgery, Worker's Hospital of Tangshan, Tangshan City, China.
Brain Tumor Research Laboratory, Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Korea.
出版信息
Brain Tumor Res Treat. 2014 Apr;2(1):22-8. doi: 10.14791/btrt.2014.2.1.22. Epub 2014 Apr 29.
BACKGROUND
To investigate the molecular basis for invasion of malignant gliomas, proteomic analysis approach was carried out using two human glioma cell lines, U87MG and U343MG-A that demonstrate different motility and invasiveness in in vitro experiments.
METHODS
High-resolution two-dimensional gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analysis were performed.
RESULTS
Nine distinct protein spots that were recognized with significant alteration between the two cell lines. Five of these protein spots were up-regulated in U87MG and four were up-regulated in U343MG-A.
CONCLUSION
Among these proteins, cathepsin D was shown to be one of the important proteins which are related with glioma invasion. However, further studies are necessary to reveal the exact role and mechanism of cathepsin D in glioma invasion.
背景
为了研究恶性胶质瘤侵袭的分子基础，采用蛋白质组学分析方法，对两种人胶质瘤细胞系U87MG和U343MG - A进行研究，这两种细胞系在体外实验中表现出不同的运动性和侵袭性。
方法
进行了高分辨率二维凝胶电泳和基质辅助激光解吸/电离飞行时间质谱分析。
结果
在两种细胞系之间识别出9个明显改变的蛋白质斑点。其中5个蛋白质斑点在U87MG中上调，4个在U343MG - A中上调。
结论
在这些蛋白质中，组织蛋白酶D被证明是与胶质瘤侵袭相关的重要蛋白质之一。然而，需要进一步研究以揭示组织蛋白酶D在胶质瘤侵袭中的确切作用和机制。
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