Li Hong, Tao Songchao, Yan Yonggang, Lv Guoyu, Gu Yifei, Luo Xiaoman, Yang Lili, Wei Jie
a College of Physical Science and Technology, Sichuan University , Chengdu 610041 , China.
J Biomater Sci Polym Ed. 2014;25(11):1194-210. doi: 10.1080/09205063.2014.926001. Epub 2014 Jun 13.
In this study, a tricalcium phosphate (TCP) and poly (amino acid) copolymer (PAA) biocomposite were fabricated for bone repair and characterized. The results show that the compressive strength of the TCP/PAA composites increased with an increase in the TCP content at TCP contents less than 40 w%. The weight loss of the composite after soaking in phosphate buffered saline for 12 weeks significantly increased with an increase in the TCP content, revealing its good degradability. In addition, the composite maintained adequate mechanical strength during the degradation period because it underwent a surface erosion process. In vitro MG63 cell culture experiments showed that the composite is non-cytotoxic and thus allows cells to adhere, proliferate and differentiate. Osteoid formation was evidenced on the composite surfaces 12 weeks after its implantation into the femoral bone of dogs. Furthermore, the composite combined directly with the host bone tissue without fibrous capsule tissue, and no inflammatory responses were found, showing the good biocompatibility of the composite. It is expected that the composite may be used for the development of bone implants for orthopaedic surgery.
在本研究中，制备了用于骨修复的磷酸三钙（TCP）与聚氨基酸共聚物（PAA）生物复合材料并对其进行了表征。结果表明，在TCP含量低于40 w%时，TCP/PAA复合材料的抗压强度随TCP含量的增加而提高。复合材料在磷酸盐缓冲盐水中浸泡12周后的失重随TCP含量的增加而显著增加，表明其具有良好的降解性。此外，该复合材料在降解期间保持了足够的机械强度，因为它经历了表面侵蚀过程。体外MG63细胞培养实验表明，该复合材料无细胞毒性，因此能使细胞黏附、增殖和分化。将该复合材料植入犬股骨12周后，在其表面有类骨质形成。此外，该复合材料直接与宿主骨组织结合，无纤维囊组织形成，也未发现炎症反应，显示出该复合材料良好的生物相容性。预计该复合材料可用于开发骨科手术用骨植入物。
