Shin Na-Rae, Shin In-Sik, Jeon Chan-Mi, Hong Ju-Mi, Oh Sei-Ryang, Hahn Kyu-Woung, Ahn Kyung-Seop
Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gun, Chungbuk 363‑883, Republic of Korea.
Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University, Daejeon 306‑791, Republic of Korea.
Mol Med Rep. 2014 Sep;10(3):1495-500. doi: 10.3892/mmr.2014.2322. Epub 2014 Jun 13.
Picrasma quassioides (D.Don) Benn. (PQ) is used in traditional medicine for the treatment of inflammatory conditions, including gastritis. This study aimed to evaluate the inhibitory effects of PQ on the inflammatory responses in mice with allergic asthma induced by ovalbumin (OVA) and in lipopolysaccharide (LPS)‑stimulated RAW264.7 cells. To induce allergic asthma, the mice underwent OVA sensitization on days 0 and 14 and then were challenged with OVA from days 21‑23. The mice were administered 15 and 30 mg/kg doses of PQ 1 h prior to the OVA challenge. The PQ treatment decreased the inflammatory cell count in the bronchoalveolar lavage fluid of the mice and reduced the levels of interleukin (IL)‑4, IL‑5, IL‑13 and immunoglobulin (Ig)E when compared with those in the OVA group. The PQ treatment also reduced the airway hyperresponsiveness induced by the OVA challenge, attenuated the recruitment of inflammatory cells and the mucus production in the airways of the mice. In the LPS‑stimulated RAW264.7 cells, the PQ treatment reduced the overexpression of inducible nitric oxide synthase (iNOS). The results indicated that PQ inhibits inflammatory responses in mice with OVA‑sensitized/challenged allergic asthma and in LPS‑stimulated RAW264.7 cells. These effects were considered to be associated with the suppression of iNOS expression. Therefore, PQ may have the potential to treat airway inflammatory diseases, including allergic asthma.
苦木（Picrasma quassioides (D.Don) Benn.，PQ）在传统医学中用于治疗包括胃炎在内的炎症性疾病。本研究旨在评估PQ对卵清蛋白（OVA）诱导的过敏性哮喘小鼠以及脂多糖（LPS）刺激的RAW264.7细胞炎症反应的抑制作用。为诱导过敏性哮喘，小鼠在第0天和第14天进行OVA致敏，然后在第21至23天用OVA进行激发。在OVA激发前1小时给小鼠分别腹腔注射15和30 mg/kg剂量的PQ。与OVA组相比，PQ治疗降低了小鼠支气管肺泡灌洗液中的炎症细胞计数，并降低了白细胞介素（IL）-4、IL-5、IL-13和免疫球蛋白（Ig）E的水平。PQ治疗还降低了OVA激发诱导的气道高反应性，减轻了小鼠气道中炎症细胞的募集和黏液分泌。在LPS刺激的RAW264.7细胞中，PQ治疗降低了诱导型一氧化氮合酶（iNOS）的过表达。结果表明，PQ抑制OVA致敏/激发的过敏性哮喘小鼠和LPS刺激的RAW264.7细胞中的炎症反应。这些作用被认为与iNOS表达的抑制有关。因此，PQ可能具有治疗包括过敏性哮喘在内的气道炎症性疾病的潜力。
