Andrews N W, Einstein M, Nussenzweig V
Department of Pathology, New York University Medical Center, New York 10016.
Am J Trop Med Hyg. 1989 Jan;40(1):46-9. doi: 10.4269/ajtmh.1989.40.46.
The surface of amastigote forms of Trypanosoma cruzi is covered by a stage-specific glycoprotein, Ssp-4. We show that Y strain-derived Ssp-4 is recognized by antibodies in sera from Chagasic patients. All 51 sera reacted with the surface of amastigotes by indirect immunofluorescence assays and immunoprecipitated Ssp-4. The human antibodies inhibited the binding of monoclonal antibodies to Ssp-4 in immunoradiometric assays, suggesting that the corresponding region of the molecule may be conserved among distinct strains of the parasite.
克氏锥虫无鞭毛体形式的表面覆盖着一种阶段特异性糖蛋白Ssp-4。我们发现，恰加斯病患者血清中的抗体可识别源自Y株的Ssp-4。通过间接免疫荧光试验和免疫沉淀Ssp-4，所有51份血清均与无鞭毛体表面发生反应。在免疫放射分析中，人源抗体抑制单克隆抗体与Ssp-4的结合，这表明该分子的相应区域在寄生虫的不同菌株中可能是保守的。
