Miyamoto-Sato Etsuko
Division of Interactome Medical Sciences, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan,
Methods Mol Biol. 2014;1164:23-32. doi: 10.1007/978-1-4939-0805-9_3.
Next-generation sequencing (NGS) has been applied to various kinds of omics studies, resulting in many biological and medical discoveries. However, high-throughput protein-protein interactome datasets derived from detection by sequencing are scarce, because protein-protein interaction analysis requires many cell manipulations to examine the interactions. The low reliability of the high-throughput data is also a problem. Here, we describe a cell-free display technology combined with NGS that can improve both the coverage and reliability of interactome datasets. This in vitro method is suitable for exploring the interactome networks of transcription factors.
下一代测序（NGS）已应用于各种组学研究，带来了许多生物学和医学上的发现。然而，通过测序检测获得的高通量蛋白质-蛋白质相互作用组数据集却很稀少，因为蛋白质-蛋白质相互作用分析需要进行许多细胞操作来检测相互作用。高通量数据的低可靠性也是一个问题。在此，我们描述了一种与NGS相结合的无细胞展示技术，它可以提高相互作用组数据集的覆盖范围和可靠性。这种体外方法适用于探索转录因子的相互作用组网络。
