Maekawa Sho, Suzuki Ayako, Sugano Sumio, Suzuki Yutaka
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba, 277-8562, Japan.
Methods Mol Biol. 2014;1164:51-65. doi: 10.1007/978-1-4939-0805-9_6.
The introduction of RNA sequencing (RNA-seq), as a direct result of rapid progression of next-generation sequencing technologies, has revolutionized the world of transcriptomics. It enables quantification of the complete set of RNA with all its isoforms in a given cell, in far greater accuracy than before. Here, we provide a step-by-step guide to the sample preparation in RNA-seq and an overview of the bioinformatic analysis that is required following the cDNA sequencing.
作为下一代测序技术快速发展的直接成果，RNA测序（RNA-seq）的引入彻底改变了转录组学领域。它能够以比以往更高的准确性对给定细胞中所有异构体的全套RNA进行定量。在这里，我们提供了RNA-seq样本制备的详细步骤指南以及cDNA测序后所需的生物信息学分析概述。
