Sousa Célia, Compère Chantal, Dreanno Catherine, Crassous Marie-Pierre, Gas Fabienne, Baus Beatrice, Perrot Hubert
Sorbonne Universités, UPMC Univ Paris 06, UMR 8235, Laboratoire Interfaces et Systèmes Electrochimiques, F-75252 Paris, France; CNRS, UMR 8235, LISE, F-75005 Paris, France; Universidade Federal do Rio de Janeiro, Instituto de Química, Av. Athos da Silveira Ramos, 149, CT, Bl. A, sala 408, Ilha da Cidade Universitária, CEP: 21.941-909 Rio de Janeiro, RJ, Brazil.
IFREMER, Centre de Brest, Laboratoire Détection, Capteurs et Mesures, F-29280 Plouzané, France.
J Microbiol Methods. 2014 Sep;104:49-54. doi: 10.1016/j.mimet.2014.05.025. Epub 2014 Jun 11.
In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cell concentration. This simple system is potentially promising for environmental monitoring purposes.
在本文中，利用高灵敏度石英晶体微天平开发了一种对有毒藻类微小亚历山大藻的简易检测方法。在性能方面，与其他传统分析工具相比，我们的免疫传感器的主要优势在于能够快速直接地检测这些活细胞。该系统需要使用一种针对微小亚历山大藻表面抗原的单克隆抗体。我们证明了能够捕获并检测整个活的且具运动性的藻类。通过对其他几种甲藻物种进行测试，也证明了该生物传感器具有高特异性。频率偏移与微小亚历山大藻细胞浓度相关。这个简易系统在环境监测方面具有潜在的应用前景。
