Xu Xiao Bing, Hu Ying, Wang Yang, Lai Chui Jin, Zhang Tian Yu
Department of Otorhinolaryngology, EYE and ENT Hospital of Fudan University, China.
Department of Otorhinolaryngology, EYE and ENT Hospital of Fudan University, China.
Auris Nasus Larynx. 2014 Dec;41(6):539-42. doi: 10.1016/j.anl.2014.05.011. Epub 2014 Jun 11.
The aim of this study was to investigate the association between laryngeal expression of claudin-3 and laryngopharyngeal reflux (LPR) in a rat reflux model.
Eight Wistar rats were divided into two groups. Four rats underwent total esophageal myectomy to induce reflux, and the remainder underwent a sham operation as a control. All animals were sacrificed 12 weeks after surgery to perform tissue histology and Western blot analysis.
Lymphocyte infiltration increased significantly in the study group in both esophageal and laryngeal samples (P=0.001, 0.002, respectively). Both esophageal and laryngeal expressions of claudin-3 were significantly lower in the study group when compared with that in the control group (P=0.045, 0.037, respectively).
The results of this study suggest that a decrease in claudin-3 could be a sensitive indicator of reflux laryngitis in rats.
本研究旨在探讨在大鼠反流模型中，紧密连接蛋白3（claudin-3）的喉部表达与喉咽反流（LPR）之间的关联。
将8只Wistar大鼠分为两组。4只大鼠接受全食管肌层切除术以诱导反流，其余大鼠接受假手术作为对照。所有动物在术后12周处死，进行组织组织学检查和蛋白质免疫印迹分析。
研究组食管和喉部样本中的淋巴细胞浸润均显著增加（分别为P = 0.001、0.002）。与对照组相比，研究组食管和喉部claudin-3的表达均显著降低（分别为P = 0.045、0.037）。
本研究结果表明，claudin-3的降低可能是大鼠反流性喉炎的一个敏感指标。
