Quintero-Villegas Maria I, Wittke Anja, Hutkins Robert
University of Nebraska Lincoln, Food Science and Technology, Lincoln, NE, USA.
Curr Microbiol. 2014 Oct;69(4):574-9. doi: 10.1007/s00284-014-0623-7. Epub 2014 Jun 14.
Cronobacter sakazakii is now recognized as an opportunistic pathogen and has been implicated in rare but severe cases of necrotizing enterocolitis, meningitis, and sepsis in neonates. The first step in bacterial pathogenesis requires that the organism adheres to host cells surfaces; therefore, agents that inhibit adherence might be useful for preventing infections. Lactoferrin, an iron binding protein found in milk, has been shown to inhibit bacterial adherence by direct interaction and disruption of bacterial surfaces. Therefore, the goal of this research was to assess the ability of two different types of bovine lactoferrin, alone and in combination with a 1:1 blend of galactooligosaccharides and polydextrose, to inhibit adherence of C. sakazakii to a HEp-2 human cell line. Results showed that the adherence of C. sakazakii was significantly reduced at a minimum lactoferrin concentration of 10 mg/ml. However, in combination with the oligosaccharide blend, no synergistic effect was observed in adherence inhibition. These results suggest that lactoferrin might interact with C. sakazakii and directly inhibit adhesion to tissue culture cells.
阪崎肠杆菌现已被确认为一种机会致病菌，与新生儿罕见但严重的坏死性小肠结肠炎、脑膜炎和败血症病例有关。细菌致病的第一步要求该生物体粘附于宿主细胞表面；因此，抑制粘附的药物可能有助于预防感染。乳铁蛋白是一种存在于牛奶中的铁结合蛋白，已被证明可通过直接相互作用和破坏细菌表面来抑制细菌粘附。因此，本研究的目的是评估两种不同类型的牛乳铁蛋白单独以及与低聚半乳糖和聚葡萄糖按1:1混合使用时抑制阪崎肠杆菌粘附人肝癌细胞系（HEp-2）的能力。结果表明，在乳铁蛋白最低浓度为10毫克/毫升时，阪崎肠杆菌的粘附显著减少。然而，与低聚糖混合物联合使用时，在粘附抑制方面未观察到协同效应。这些结果表明，乳铁蛋白可能与阪崎肠杆菌相互作用并直接抑制其对组织培养细胞的粘附。
