用于蛋白质药物递送的具有高包封效率的聚合物微胶囊的微流控辅助工程。

Microfluidics-assisted engineering of polymeric microcapsules with high encapsulation efficiency for protein drug delivery.

作者信息

Pessi Jenni, Santos Hélder A, Miroshnyk Inna, Weitz David A, Mirza Sabiruddin

机构信息

Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland; School of Engineering and Applied Science, Department of Physics, Harvard University, Cambridge, MA 02138, USA.

Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland.

出版信息

Int J Pharm. 2014 Sep 10;472(1-2):82-7. doi: 10.1016/j.ijpharm.2014.06.012. Epub 2014 Jun 10.

DOI:10.1016/j.ijpharm.2014.06.012

PMID:24928131

Abstract

In this study, microfluidic technology was employed to develop protein formulations. The microcapsules were produced with a biphasic flow to create water-oil-water (W/O/W) double emulsion droplets with ultrathin shells. Optimized microcapsule formulations containing 1% (w/w) bovine serum albumin (BSA) in the inner phase were prepared with poly(vinyl alcohol), polycaprolactone and polyethylene glycol. All the particles were found to be intact and with a particle size of 23-47 μm. Furthermore, the particles were monodisperse, non-porous and stable up to 4 weeks. The encapsulation efficiency of BSA in the microcapsules was 84%. The microcapsules released 30% of their content within 168 h. This study demonstrates that microfluidics is a powerful technique for engineering formulations for therapeutic proteins.

摘要

在本研究中，采用微流控技术来开发蛋白质制剂。微胶囊通过双相流制备，以形成具有超薄壳层的水包油包水（W/O/W）双重乳液滴。使用聚乙烯醇、聚己内酯和聚乙二醇制备了在内相中含有1%（w/w）牛血清白蛋白（BSA）的优化微胶囊制剂。发现所有颗粒均完整无损，粒径为23 - 47μm。此外，这些颗粒是单分散的、无孔的，并且在长达4周的时间内保持稳定。微胶囊中BSA的包封效率为84%。微胶囊在168小时内释放了其内容物的30%。本研究表明，微流控技术是一种用于工程化治疗性蛋白质制剂的强大技术。