Deckers Markus, Balleininger Martina, Vukotic Milena, Römpler Katharina, Bareth Bettina, Juris Lisa, Dudek Jan
Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.
Department of Cellular Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.
FEBS Lett. 2014 Aug 25;588(17):2985-92. doi: 10.1016/j.febslet.2014.06.006. Epub 2014 Jun 10.
The mitochondrial respiratory chain is essential for the conversion of energy derived from the oxidation of metabolites into the membrane potential, which drives the synthesis of ATP. The electron transporting complexes bc1 complex and the cytochrome c oxidase assemble into large supercomplexes, allowing efficient energy transduction. Currently, we have only limited information about what determines the structure of the supercomplex. Here, we characterize Aim24 in baker's yeast as a protein, which is integrated in the mitochondrial inner membrane and is required for the structural integrity of the supercomplex. Deletion of AIM24 strongly affects activity of the respiratory chain and induces a growth defect on non-fermentable medium. Our data indicate that Aim24 has a function in stabilizing the respiratory chain supercomplexes.
线粒体呼吸链对于将代谢物氧化产生的能量转化为膜电位至关重要，而膜电位驱动ATP的合成。电子传递复合物bc1复合物和细胞色素c氧化酶组装成大型超级复合物，从而实现高效的能量转导。目前，我们对于决定超级复合物结构的因素了解有限。在此，我们将酿酒酵母中的Aim24鉴定为一种整合在线粒体内膜中的蛋白质，它是超级复合物结构完整性所必需的。删除AIM24会强烈影响呼吸链的活性，并在非发酵培养基上诱导生长缺陷。我们的数据表明，Aim24在稳定呼吸链超级复合物方面具有功能。
