Department of Environmental Engineering, Yonsei University, Wonju, 220-710, South Korea.
Environ Sci Pollut Res Int. 2014 Nov;21(21):12270-8. doi: 10.1007/s11356-014-3157-4. Epub 2014 Jun 15.
This study evaluated the toxicity of herbicide atrazine, along with its bioaccumulation and biodegradation in the green microalga Chlamydomonas mexicana. At low concentration (10 μg L(-1)), atrazine had no profound effect on the microalga, while higher concentrations (25, 50, and 100 μg L(-1)) imposed toxicity, leading to inhibition of cell growth and chlorophyll a accumulation by 22 %, 33 %, and 36 %, and 13 %, 24 %, and 27 %, respectively. Atrazine 96-h EC50 for C. mexicana was estimated to be 33 μg L(-1). Microalga showed a capability to accumulate atrazine in the cell and to biodegrade the cell-accumulated atrazine resulting in 14-36 % atrazine degradation at 10-100 μg L(-1). Increasing atrazine concentration decreased the total fatty acids (from 102 to 75 mg g(-1)) and increased the unsaturated fatty acid content in the microalga. Carbohydrate content increased gradually with the increase in atrazine concentration up to 15 %. This study shows that C. mexicana has the capability to degrade atrazine and can be employed for the remediation of atrazine-contaminated streams.
本研究评估了除草剂莠去津的毒性及其在绿藻墨西哥拟衣藻中的生物积累和生物降解作用。在低浓度（10μg/L）时，莠去津对微藻没有明显影响，而较高浓度（25、50 和 100μg/L）则会产生毒性，导致细胞生长抑制和叶绿素 a 积累分别减少 22%、33%和 36%，以及 13%、24%和 27%。墨西哥拟衣藻对莠去津的 96 小时 EC50 估计为 33μg/L。微藻表现出在细胞内积累莠去津的能力，并能生物降解细胞内积累的莠去津，导致在 10-100μg/L 时莠去津降解率为 14-36%。莠去津浓度的增加降低了总脂肪酸（从 102 到 75mg/g），并增加了微藻中不饱和脂肪酸的含量。随着莠去津浓度的增加，碳水化合物含量逐渐增加，最高可达 15%。本研究表明，墨西哥拟衣藻具有降解莠去津的能力，可用于受莠去津污染的溪流的修复。
