Boldbaatar Delgerbat, El-Seedi Hesham R, Findakly Meriana, Jabri Safa, Javzan Batkhuu, Choidash Battsetseg, Göransson Ulf, Hellman Björn
Department of Medicinal Chemistry, Division of Pharmacognosy, Uppsala University, Box 574, SE-751 23 Uppsala, Sweden; School of Biology and Biotechnology, National University of Mongolia, Ulaanbaatar 46, Mongolia.
Department of Medicinal Chemistry, Division of Pharmacognosy, Uppsala University, Box 574, SE-751 23 Uppsala, Sweden; Department of Chemistry, Faculty of Science, El-Menoufia University, 32512 Shebin El-Kom, Egypt.
J Ethnopharmacol. 2014 Aug 8;155(1):599-606. doi: 10.1016/j.jep.2014.06.005. Epub 2014 Jun 10.
Leptopyrum fumarioides has been used in the traditional medicine of Mongolia for the treatment of various diseases, including drug intoxications. However, since there is only sparse information about its chemistry, active components, and pharmacological and toxicological effects, the major aim of the present study employing mouse lymphoma cells was to evaluate the genotoxic and antigenotoxic/antioxidative effects of extracts and components isolated from this plant.
A crude methanol extract was separated into three different sub-extracts: dichloromethane, n-butanol, and water. The major constituent of the n-butanol extract, i.e., the flavone luteolin-7-O-glucoside and a mixture of the most abundant compounds in the dichloromethane sub-extract were then isolated. DNA damage was evaluated using the comet assay; the antioxidant activity was evaluated using the DPPH radical scavenging assay.
The crude methanol extract, the dichloromethane sub-extract and the mixture of compounds isolated from the latter fraction, increased the level of DNA damage after three hours of exposure. In contrast, no increase in DNA damage was observed in the cells that had been exposed to the n-butanol and water sub-extracts, or to the pure flavone. When non-DNA damaging concentrations of extracts and compounds were tested together with the DNA damaging agent catechol, all sub-extracts were found to reduce the catechol-induced DNA damage (the flavone was then found to be the most effective protective agent). The n-butanol sub-extract and the flavone were also found to have the most prominent antioxidative effects.
Based on the results from the present study, components in Leptopyrum fumarioides were found to protect the DNA damage induced by catechol, probably by acting as potent antioxidants.
烟堇叶碱毛茛在蒙古传统医学中用于治疗包括药物中毒在内的各种疾病。然而，由于关于其化学、活性成分以及药理和毒理作用的信息稀少，本研究采用小鼠淋巴瘤细胞的主要目的是评估从该植物中分离得到的提取物和成分的遗传毒性以及抗遗传毒性/抗氧化作用。
将粗甲醇提取物分离为三种不同的亚提取物：二氯甲烷、正丁醇和水。然后分离出正丁醇提取物的主要成分，即黄酮木犀草素 -7-O-葡萄糖苷以及二氯甲烷亚提取物中含量最丰富的化合物混合物。使用彗星试验评估DNA损伤；使用DPPH自由基清除试验评估抗氧化活性。
粗甲醇提取物、二氯甲烷亚提取物以及从后一组分中分离得到的化合物混合物，在暴露三小时后增加了DNA损伤水平。相比之下，在暴露于正丁醇和水亚提取物或纯黄酮的细胞中未观察到DNA损伤增加。当将非DNA损伤浓度的提取物和化合物与DNA损伤剂儿茶酚一起测试时，发现所有亚提取物均能减少儿茶酚诱导的DNA损伤（随后发现黄酮是最有效的保护剂）。还发现正丁醇亚提取物和黄酮具有最显著的抗氧化作用。
基于本研究结果，发现烟堇叶碱毛茛中的成分可能通过作为强效抗氧化剂来保护儿茶酚诱导的DNA损伤。
