Li Yueying, Charif Naceur, Mainard Didier, Bensoussan Danièle, Stoltz Jean-François, de Isla Natalia
CNRS, UMR 7365 IMoPA, Vandœuvre-lès-Nancy, France School of Medicine, Wuhan University, China.
CNRS, UMR 7365 IMoPA, Vandœuvre-lès-Nancy, France Faculty of Medicine, UL, Vandœuvre-lès-Nancy, France.
Biomed Mater Eng. 2014;24(1 Suppl):47-52. doi: 10.3233/BME-140973.
While mesenchymal stem cells represent an interesting cell source for regenerative medicine, several points have to be investigated to improve their use in clinical, and in particular in the elderly population. This work studied the proliferation capacity of mesenchymal stem cells isolated from human bone marrow in function of donor's age. Doubling time after in vitro culture, clonogenicity and phenotype were analyzed in 17 samples ranging from 3 to 85 years old (mean 47 ± 27). Results showed an increase in the doubling time for cell coming from old donor compared to cells coming from young ones. This was accompanied by a decrease in clonogenicity while no changes were observe in cell phenotype. In conclusion, this study showed an effect of donor's age on the proliferation capacity of mesenchymal stem cells isolated from bone marrow that was correlated to a decrease in clonogenicity. The comprehension of molecular mechanism involved in this process could help to improve the clinical application of mesenchymal stem cells.
虽然间充质干细胞是再生医学中一种引人关注的细胞来源，但为了改善其在临床中的应用，尤其是在老年人群中的应用，仍有几个要点需要研究。这项工作研究了从人骨髓中分离的间充质干细胞的增殖能力与供体年龄的关系。对17个年龄在3至85岁（平均47±27岁）的样本进行了体外培养后的倍增时间、克隆形成能力和表型分析。结果显示，与年轻供体来源的细胞相比，老年供体来源的细胞倍增时间增加。这伴随着克隆形成能力的下降，而细胞表型未观察到变化。总之，本研究表明供体年龄对从骨髓中分离的间充质干细胞的增殖能力有影响，且这与克隆形成能力的下降相关。对这一过程中涉及的分子机制的理解有助于改善间充质干细胞的临床应用。
