Mendoza-Porras Omar, Botwright Natasha A, McWilliam Sean M, Cook Mathew T, Harris James O, Wijffels Gene, Colgrave Michelle L
CSIRO Food Futures Flagship, 5 Julius Avenue, North Ryde, New South Wales 2113, Australia; CSIRO Animal, Food and Health Sciences, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Queensland 4067, Australia; School of Biological Sciences, Flinders University, GPO 2100, South Australia 5001, Australia; Australian Seafood Cooperative Research Centre, Science Park Adelaide, Laffer Drive, Bedford Park, South Australia 5042, Australia.
CSIRO Food Futures Flagship, 5 Julius Avenue, North Ryde, New South Wales 2113, Australia; CSIRO Animal, Food and Health Sciences, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Queensland 4067, Australia.
J Proteomics. 2014 Aug 28;108:337-53. doi: 10.1016/j.jprot.2014.06.001. Epub 2014 Jun 12.
Aside from their critical role in reproduction, abalone gonads serve as an indicator of sexual maturity and energy balance, two key considerations for effective abalone culture. Temperate abalone farmers face issues with tank restocking with highly marketable abalone owing to inefficient spawning induction methods. The identification of key proteins in sexually mature abalone will serve as the foundation for a greater understanding of reproductive biology. Addressing this knowledge gap is the first step towards improving abalone aquaculture methods. Proteomic profiling of female and male gonads of greenlip abalone, Haliotis laevigata, was undertaken using liquid chromatography-mass spectrometry. Owing to the incomplete nature of abalone protein databases, in addition to searching against two publicly available databases, a custom database comprising genomic data was used. Overall, 162 and 110 proteins were identified in females and males respectively with 40 proteins common to both sexes. For proteins involved in sexual maturation, sperm and egg structure, motility, acrosomal reaction and fertilization, 23 were identified only in females, 18 only in males and 6 were common. Gene ontology analysis revealed clear differences between the female and male protein profiles reflecting a higher rate of protein synthesis in the ovary and higher metabolic activity in the testis.
A comprehensive mass spectrometry-based analysis was performed to profile the abalone gonad proteome providing the foundation for future studies of reproduction in abalone. Key proteins involved in both reproduction and energy balance were identified. Genomic resources were utilised to build a database of molluscan proteins yielding >60% more protein identifications than in a standard workflow employing public protein databases.
除了在繁殖中发挥关键作用外，鲍鱼性腺还可作为性成熟和能量平衡的指标，这是有效养殖鲍鱼时的两个关键考量因素。由于产卵诱导方法效率低下，温带地区的鲍鱼养殖户在使用具有高市场价值的鲍鱼进行池塘补栏时面临问题。鉴定性成熟鲍鱼中的关键蛋白质将为更深入了解生殖生物学奠定基础。填补这一知识空白是改进鲍鱼养殖方法的第一步。利用液相色谱 - 质谱联用技术对绿唇鲍（Haliotis laevigata）的雌性和雄性性腺进行了蛋白质组分析。由于鲍鱼蛋白质数据库不完善，除了在两个公开可用数据库中进行搜索外，还使用了一个包含基因组数据的定制数据库。总体而言，在雌性和雄性中分别鉴定出162种和110种蛋白质，其中有40种蛋白质是两性共有的。对于参与性成熟、精子和卵子结构、活力、顶体反应和受精的蛋白质，仅在雌性中鉴定出23种，仅在雄性中鉴定出18种，两性共有6种。基因本体分析揭示了雌性和雄性蛋白质谱之间的明显差异，反映出卵巢中蛋白质合成速率较高，而睾丸中的代谢活性较高。
进行了基于质谱的全面分析以描绘鲍鱼性腺蛋白质组，为未来鲍鱼繁殖研究奠定了基础。鉴定出了参与繁殖和能量平衡的关键蛋白质。利用基因组资源构建了软体动物蛋白质数据库，与使用公共蛋白质数据库的标准流程相比，蛋白质鉴定数量增加了60%以上。
