Kim Mi Ae, Kim Tae Ha, Lee Sora, Nam Bo-Hye, Lee Jung Sick, Jang Wonhee, Sohn Young Chang
Department of Marine Molecular Bioscience, Gangneung-Wonju National University, Gangneung, Gangwon, 25457, Republic of Korea.
The East Coast Research Institute of Life Science, Gangneung-Wonju National University, Gangneung, Gangwon, 25457, Republic of Korea.
Genes Genomics. 2020 Oct;42(10):1179-1188. doi: 10.1007/s13258-020-00983-z. Epub 2020 Aug 17.
There is now abundant information on genes involved in molluscan oogenesis and their associations with ovarian development. However, few studies have investigated the ovarian transcriptome of Pacific abalone (Haliotis discus hannai).
The objective of this study was to identify genes related to ovarian development and maturation in Pacific abalone utilizing RNA-sequencing (RNA-seq) and to verify the genes most relevant to different stages of maturation.
RNA samples from the ovarian tissues of sexually immature and mature abalone were used to construct cDNA libraries, which were paired-end sequenced on an Illumina HiSeq 2500 platform. Reads from individual samples (unigenes) were aligned to reference transcriptome databases for identification of differentially expressed genes (DEGs) between immature and mature ovarian libraries. Reverse transcription-quantitative polymerase chain reaction was used to verify the RNA-seq data.
A total of 8779 unigenes were obtained from the ovaries of immature and mature abalone, with a total length of 3323,279 bp and an average length of 379 bp per gene. Gene ontology analysis assigned 5860 unigenes to biological processes, 855 to cellular components, and 1352 to molecular functions. Overall, 470 DEGs were identified, including 213 and 257 genes down-regulated and up-regulated in mature abalone, respectively. Among these, 13 relevant transcripts, including VTG1 and FZD7, were significantly highly expressed in the ovaries of mature abalone (p < 0.05, fold change > 2).
This H. discus hannai ovary transcriptome provides molecular targets to better understand ovarian development, oogenesis, and sexual maturation, and to enhance Pacific abalone production.
目前，关于参与软体动物卵子发生的基因及其与卵巢发育的关联已有丰富信息。然而，对太平洋鲍鱼（皱纹盘鲍）卵巢转录组的研究较少。
本研究旨在利用RNA测序（RNA-seq）鉴定与太平洋鲍鱼卵巢发育和成熟相关的基因，并验证与不同成熟阶段最相关的基因。
使用性未成熟和成熟鲍鱼卵巢组织的RNA样本构建cDNA文库，在Illumina HiSeq 2500平台上进行双末端测序。将来自各个样本的读段（单基因）与参考转录组数据库进行比对，以鉴定未成熟和成熟卵巢文库之间的差异表达基因（DEG）。采用逆转录定量聚合酶链反应验证RNA-seq数据。
从未成熟和成熟鲍鱼的卵巢中总共获得了8779个单基因，全长3323279 bp，每个基因平均长度为379 bp。基因本体分析将5860个单基因归类到生物学过程，855个归类到细胞组分，1352个归类到分子功能。总体而言，共鉴定出470个DEG，其中成熟鲍鱼中分别有213个和257个基因下调和上调。其中，包括VTG1和FZD7在内的13个相关转录本在成熟鲍鱼卵巢中显著高表达（p < 0.05，变化倍数> 2）。
该皱纹盘鲍卵巢转录组提供了分子靶点，有助于更好地理解卵巢发育、卵子发生和性成熟，并提高太平洋鲍鱼的产量。
