Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
Bioresour Technol. 2014 Aug;166:620-4. doi: 10.1016/j.biortech.2014.05.098. Epub 2014 Jun 2.
Microalgal lipid with a high free fatty acid (FFA) content was directly extracted from Chlorella vulgaris, using SDBS, in an acid-catalyzed hot-water extraction process. The total fatty acid content of C. vulgaris was 296.0 mg/g cell. Under the 1.0% sulfuric acid, 0.4% SDBS conditions, the FFA content of the lipid increased to 96.7%, and the lipid-extraction yield was 248.4 mg/g cell. Under the 2.0% sulfuric acid, 0.2% SDBS conditions, the FFA content of the lipid was 96.1%, and the lipid-extraction yield was 266.0mg/g cell. Whereas the FAME content of the microalgal lipid extracted by hexane-methanol was 76.4% at the 10.0% sulfuric acid concentration, the FAME content of the high-FFA microalgal lipid was increased to 70.1% at a sulfuric acid concentration of only 0.1%. By combined sulfuric acid/SDBS treatment, high-FFA microalgal lipid was extracted in large yields; moreover, the amount of catalyst was remarkably reduced in the esterification of FFA.
从普通小球藻中直接提取高游离脂肪酸（FFA）含量的微藻油脂，采用 SDBS，在酸催化热水萃取过程中。小球藻的总脂肪酸含量为 296.0mg/g 细胞。在 1.0%硫酸、0.4%SDBS 条件下，油脂的 FFA 含量增加到 96.7%，油脂提取率为 248.4mg/g 细胞。在 2.0%硫酸、0.2%SDBS 条件下，油脂的 FFA 含量为 96.1%，油脂提取率为 266.0mg/g 细胞。而用正己烷-甲醇提取的微藻油脂的 FAME 含量在 10.0%硫酸浓度下为 76.4%，高 FFA 微藻油脂的 FAME 含量在硫酸浓度仅为 0.1%时增加到 70.1%。通过硫酸/SDBS 联合处理，可以以高得率提取高 FFA 微藻油脂；此外，在 FFA 的酯化中显著减少了催化剂的用量。
