Biomass and Waste to Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon, 34129, Republic of Korea.
Moojin Machinetool, 53-11 Hanamsandancheonbyeonjwa-ro, Gwangsan-gu, Gwangju, 62213, Republic of Korea.
Appl Biochem Biotechnol. 2021 Feb;193(2):319-334. doi: 10.1007/s12010-020-03426-3. Epub 2020 Sep 21.
The feasibility of surfactants for enhancement of extraction efficiencies in wet oil extraction through an acidic hydrothermal process was evaluated. Three different types of surfactants were tested: anionic (SDBS and SDS), cationic (CTAB and MBC), and non-ionic (IGEPAL CA-210 and Tween 60). The total fatty acid content of Chlorella vulgaris was 291.0 mg/g cell. Under the no-surfactant condition, the oil-extraction yield of the acidic hydrothermal extraction was 75.5%. The addition of SDBS and MBC at the 0.4% concentration showed enhanced oil-extraction performance, 85.4 and 85.7% yields, respectively. CTAB and Tween 60 showed low extraction yields, less than 43.0%. SDS and IGEPAL CA-210 showed high oil-extraction yields, higher, in fact, than the initial fatty acid content, due to surfactant partitioning into microalgal oil. With increasing surfactant concentration, the oil-extraction yields of CTAB decreased, those of IGEPAL CA-210 gradually increased, and those of SDBS increased and then decreased again. The best performance, an oil-extraction yield of 95.6%, was observed under the 0.2% SDBS, 120 °C, 1 h condition. Although IGEPAL CA-210 showed the high net oil-extraction yield of 98.3% at the 0.6% surfactant concentration, 61.2% of surfactant was partitioned into oil. Graphical abstract.
评估了表面活性剂在酸性水热萃取中提高萃取效率的可行性。测试了三种不同类型的表面活性剂：阴离子（SDBS 和 SDS）、阳离子（CTAB 和 MBC）和非离子（IGEPAL CA-210 和 Tween 60）。小球藻的总脂肪酸含量为 291.0mg/g 细胞。在无表面活性剂条件下，酸性水热萃取的油萃取率为 75.5%。在 0.4%浓度下添加 SDBS 和 MBC 表现出增强的油萃取性能，分别为 85.4%和 85.7%的收率。CTAB 和 Tween 60 表现出低的萃取收率，低于 43.0%。SDS 和 IGEPAL CA-210 表现出高的油萃取收率，实际上高于初始脂肪酸含量，这是由于表面活性剂分配到微藻油中。随着表面活性剂浓度的增加，CTAB 的油萃取收率降低，IGEPAL CA-210 的油萃取收率逐渐增加，而 SDBS 的油萃取收率先增加后又降低。在 0.2%SDBS、120°C、1h 的条件下，观察到最佳性能，油萃取收率为 95.6%。尽管 IGEPAL CA-210 在 0.6%表面活性剂浓度下表现出 98.3%的高净油萃取收率，但有 61.2%的表面活性剂分配到油相中。
