Chen Yan-Zhi, Liu Dan, Zhao Yu-Xia, Wang He-Tong, Gao Ya, Chen Ying
Department of Radiotherapy, The Fourth Affiliated Hospital of China Medical University, Chongshan East No 4, Huanggu District, Liaoning, Shenyang, 110032, People's Republic of China.
Tumour Biol. 2014 Sep;35(9):9201-10. doi: 10.1007/s13277-014-2180-x. Epub 2014 Jun 15.
This meta-analysis of published cohort studies was conducted to evaluate whether promoter methylation of the secreted frizzled-related protein 1 (SFRP1) gene contributes to colorectal carcinogenesis. The Web of Science (1945 ~ 2013), the Cochrane Library Database (Issue 12, 2013), PubMed (1966 ~ 2013), EMBASE (1980 ~ 2013), CINAHL (1982 ~ 2013), and the Chinese Biomedical Database (CBM) (1982 ~ 2013) were searched without language restrictions. Meta-analysis was conducted using the STATA 12.0 software. We calculated odds ratio (OR) and its 95 % confidence interval (95 % CI) to estimate the correlations between SFRP1 promoter methylation and colorectal carcinogenesis. In the present meta-analysis, 8 cohort studies with a total of 942 patients with colorectal cancer (CRC) were included. The pooled results revealed that the frequency of SFRP1 promoter methylation in cancer tissues were significantly higher than those of normal, adjacent, and benign tissues (cancer tissues vs. normal tissues: OR = 31.49, 95 % CI = 17.57 ~ 56.44, P < 0.001; cancer tissues vs. adjacent tissues: OR = 5.95, 95 % CI 3.12 ~ 10.00, P < 0.001; cancer tissues vs. benign tissues: OR = 3.01, 95 % CI 1.72 ~ 5.27, P < 0.001; respectively). Furthermore, ethnicity-stratified analysis indicated that SFRP1 promoter methylation was strongly correlated with colorectal carcinogenesis among both Asians and Caucasians (all P < 0.05). Our findings provide empirical evidence that SFRP1 promoter methylation may be correlated with the pathogenesis of CRC.
本项已发表队列研究的荟萃分析旨在评估分泌型卷曲相关蛋白1（SFRP1）基因的启动子甲基化是否与结直肠癌发生有关。检索了科学网（1945年至2013年）、考克兰图书馆数据库（2013年第12期）、PubMed（1966年至2013年）、EMBASE（1980年至2013年）、护理学与健康领域数据库（CINAHL，1982年至2013年）以及中国生物医学数据库（CBM，1982年至2013年），检索无语言限制。使用STATA 12.0软件进行荟萃分析。我们计算比值比（OR）及其95%置信区间（95%CI）以估计SFRP1启动子甲基化与结直肠癌发生之间的相关性。在本荟萃分析中，纳入了8项队列研究，共942例结直肠癌（CRC）患者。汇总结果显示，癌组织中SFRP1启动子甲基化频率显著高于正常组织、癌旁组织和良性组织（癌组织与正常组织比较：OR = 31.49，95%CI = 17.57至56.44，P < 0.001；癌组织与癌旁组织比较：OR = 5.95，95%CI 3.12至10.00，P < 0.001；癌组织与良性组织比较：OR = 3.01，95%CI 1.72至5.27，P < 0.001；分别）。此外，按种族分层分析表明SFRP1启动子甲基化在亚洲人和白种人中均与结直肠癌发生密切相关（所有P < 0.05）。我们的研究结果提供了经验证据，表明SFRP1启动子甲基化可能与CRC的发病机制相关。
