Sow S L S, Khoo G, Chong L K, Smith T J, Harrison P L, Ong H K A
Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, Kampar, 31900, Perak, Malaysia.
World J Microbiol Biotechnol. 2014 Oct;30(10):2645-53. doi: 10.1007/s11274-014-1687-z. Epub 2014 Jun 15.
In a previous study, notable differences of several physicochemical properties, as well as the community structure of ammonia oxidizing bacteria as judged by 16S rRNA gene analysis, were observed among several disused tin-mining ponds located in the town of Kampar, Malaysia. These variations were associated with the presence of aquatic vegetation as well as past secondary activities that occurred at the ponds. Here, methane oxidizing bacteria (MOB), which are direct participants in the nutrient cycles of aquatic environments and biological indicators of environmental variations, have been characterised via analysis of pmoA functional genes in the same environments. The MOB communities associated with disused tin-mining ponds that were exposed to varying secondary activities were examined in comparison to those in ponds that were left to nature. Comparing the sequence and phylogenetic analysis of the pmoA clone libraries at the different ponds (idle, lotus-cultivated and post-aquaculture), we found pmoA genes indicating the presence of type I and type II MOB at all study sites, but type Ib sequences affiliated with the Methylococcus/Methylocaldum lineage were most ubiquitous (46.7 % of clones). Based on rarefaction analysis and diversity indices, the disused mining pond with lotus culture was observed to harbor the highest richness of MOB. However, varying secondary activity or sample type did not show a strong variation in community patterns as compared to the ammonia oxidizers in our previous study.
在之前的一项研究中，位于马来西亚甘榜镇的几个废弃锡矿池塘在若干物理化学性质以及通过16S rRNA基因分析判断的氨氧化细菌群落结构方面存在显著差异。这些差异与水生植被的存在以及池塘过去发生的次生活动有关。在此，通过分析相同环境中的pmoA功能基因，对甲烷氧化细菌（MOB）进行了表征，甲烷氧化细菌是水生环境营养循环的直接参与者以及环境变化的生物指标。与自然状态的池塘相比，对经历了不同次生活动的废弃锡矿池塘相关的MOB群落进行了研究。通过比较不同池塘（闲置、种植莲花和水产养殖后）的pmoA克隆文库的序列和系统发育分析，我们发现在所有研究地点都存在表明I型和II型MOB存在的pmoA基因，但与甲基球菌/甲基暖菌谱系相关的Ib型序列最为普遍（占克隆的46.7%）。基于稀疏分析和多样性指数，观察到种植莲花的废弃矿塘中MOB的丰富度最高。然而，与我们之前研究中的氨氧化菌相比，不同的次生活动或样本类型在群落模式上并未表现出强烈变化。
