Yang Yuyin, Zhao Qun, Cui Yahui, Wang Yilin, Xie Shuguang, Liu Yong
State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
Microb Ecol. 2016 Jan;71(1):9-17. doi: 10.1007/s00248-015-0667-7. Epub 2015 Aug 30.
Aerobic methane-oxidizing bacteria (MOB) play a crucial role in mitigating the methane emission from lake ecosystems to the atmosphere. However, the distribution of methanotrophic community in shallow and eutrophic lake and its influential factors remain essentially unclear. The present study investigated sediment methanotrophic microorganisms at different sites in eutrophic freshwater Dianchi Lake (China) in two different seasons. The abundance, diversity, and structure of sediment methanotrophic community showed a profound spatial and seasonal variation. The pmoA gene copy number in lake sediments ranged from 8.71 ± 0.49 × 10(4) to 2.09 ± 0.03 × 10(7) copies per gram of dry sediment. Sediment methanotrophic communities were composed of Methylococcus and Methylobacter (type I methanotrophs) and Methylosinus (type II methanotrophs), while type I MOB usually outnumbered type II MOB. Moreover, ammonia nitrogen was found to be a potential determinant of methanotrophic community structure in Dianchi Lake.
好氧甲烷氧化细菌（MOB）在减轻湖泊生态系统向大气排放甲烷方面发挥着关键作用。然而，富营养浅水湖泊中甲烷营养群落的分布及其影响因素仍基本不清楚。本研究调查了中国富营养化淡水滇池不同季节不同位点的沉积物甲烷营养微生物。沉积物甲烷营养群落的丰度、多样性和结构呈现出显著的空间和季节变化。湖泊沉积物中pmoA基因拷贝数范围为每克干沉积物8.71±0.49×10⁴至2.09±0.03×10⁷个拷贝。沉积物甲烷营养群落由甲基球菌属和甲基杆菌属（I型甲烷营养菌）以及甲基弯曲菌属（II型甲烷营养菌）组成，而I型甲烷氧化细菌的数量通常多于II型。此外，氨氮被发现是滇池甲烷营养群落结构的一个潜在决定因素。
