Zhou Haifeng, Lan Tianqing, Dien Bruce S, Hector Ronald E, Zhu J Y
School of Chemistry Chemical Eng., South China University Technol., Guangzhou, China; USDA Forest Service, Forest Products Laboratory, Madison, WI.
Biotechnol Prog. 2014 Sep-Oct;30(5):1076-83. doi: 10.1002/btpr.1937. Epub 2014 Jul 16.
The performances of five yeast strains under three levels of toxicity were evaluated using hydrolysates from lodgepole pine pretreated by Sulfite Pretreatment to Overcome the Recalcitrance of Lignocelluloses (SPORL). The highest level of toxicity was represented by the whole pretreated biomass slurry, while intermediate toxicity was represented by the hydrolysate with partial loading of pretreatment spent liquor. The zero toxicity was represented using the enzymatic hydrolysate produced from thoroughly washed SPORL lodgepole pine solids. The results indicate that strains D5A and YRH400 can tolerate the whole pretreated biomass slurry to produce 90.1 and 73.5% theoretical ethanol yield. Strains Y1528, YRH403, and FPL450 did not grow in whole hydrolysate cultures and were observed to have lower ethanol productivities than D5A and YRH400 on the hydrolysate with intermediate toxicity. Both YRH400 and YRH403 were genetically engineered for xylose fermentation but were not able to consume xylose efficiently in hydrolysate.
使用经亚硫酸盐预处理以克服木质纤维素顽固性（SPORL）的黑松水解产物，评估了五种酵母菌株在三种毒性水平下的性能。最高毒性水平由整个预处理生物质浆料表示，而中等毒性由含有部分预处理废液的水解产物表示。零毒性使用由彻底洗涤的SPORL黑松固体产生的酶解水解产物表示。结果表明，菌株D5A和YRH400能够耐受整个预处理生物质浆料，以产生90.1%和73.5%的理论乙醇产率。菌株Y1528、YRH403和FPL450在全水解产物培养物中不生长，并且在中等毒性的水解产物上观察到其乙醇生产率低于D5A和YRH400。YRH400和YRH403都经过基因工程改造用于木糖发酵，但在水解产物中不能有效地消耗木糖。
