Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing, China; USDA Forest Service, Forest Products Lab, Madison, WI 53719, USA.
Dept. Civil Environ. Eng., Hong Kong Polytechnic University, Kowloon, Hong Kong; USDA Forest Service, Forest Products Lab, Madison, WI 53719, USA.
Bioresour Technol. 2014 Jul;164:331-7. doi: 10.1016/j.biortech.2014.04.102. Epub 2014 May 9.
Sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL) was applied to an empty fruit bunches (EFB) for ethanol production. SPORL facilitated delignification through lignin sulfonation and dissolution of xylan to result in a highly digestible substrate. The pretreated whole slurry was enzymatically saccharified at a solids loading of 18% using a relatively low cellulase loading of 15 FPU/g glucan and simultaneously fermented without detoxification using Saccharomyces cerevisiae of YRH400. An ethanol yield of 217 L/tonne EFB was achieved at titer of 32 g/L. Compared with literature studies, SPORL produced high ethanol yield and titer with much lower cellulase loading without detoxification.
亚硫酸盐预处理克服木质纤维素的顽固性（SPORL）被应用于生产乙醇的空果串（EFB）。SPORL 通过木质素磺化和木聚糖的溶解促进脱木质素，从而得到高可消化的底物。预处理后的整个浆液在固含量为 18%的条件下用相对较低的纤维素酶用量（15 FPU/g 葡聚糖）进行酶解，并同时使用 YRH400 的酿酒酵母进行发酵，无需解毒。在 32 g/L 的浓度下，每吨 EFB 的乙醇产量达到 217 升。与文献研究相比，SPORL 在无需解毒的情况下，用较低的纤维素酶用量就产生了较高的乙醇得率和浓度。
