ACS Nano. 2014 Jul 22;8(7):6644-54. doi: 10.1021/nn502386j.
The facile loading of sensitive and fragile biomacromolecules, such as glucose oxidase, hemoglobin, and ribonucleic acid (RNA), via synthetic vehicles directly in pure aqueous media is an important technical challenge. Inspired by the nucleus pore complex that connects the cell nucleus and the cytoplasm across the nuclear envelope, here we describe the development of a kind of polymeric nuclear envelope-like vesicle (NEV) to address this problem. The NEV is tailored to form the polymer pore complex (70 nm, similar to a nucleus pore complex) within the vesicle membrane based on nanophase segregation, which is confirmed via fluorescence spectrometry and dynamic light scattering (DLS) during self-assembly. This pH-triggered polymer pore complex can mediate the transportation of biomacromolecules across the vesicle membrane. Moreover, the NEVs facilitate the natural consecutive enzyme-catalyzed reactions via the H(+) sponge effect. This simple strategy might also be extended for mimicking other synthetic cell organelles.
在纯水溶液中通过合成载体直接将敏感和脆弱的生物大分子（如葡萄糖氧化酶、血红蛋白和核糖核酸（RNA））易于负载是一个重要的技术挑战。受核孔复合物的启发，核孔复合物将细胞核和细胞质穿过核膜连接起来，在这里我们描述了一种聚合物核膜样囊泡（NEV）的开发，以解决这个问题。根据纳米相分离，NEV 被定制为在囊泡膜内形成聚合物孔复合物（70nm，类似于核孔复合物），这在自组装过程中通过荧光光谱法和动态光散射（DLS）得到证实。这种 pH 触发的聚合物孔复合物可以介导生物大分子穿过囊泡膜的运输。此外，NEVs 通过 H(+) 海绵效应促进天然连续的酶催化反应。这种简单的策略也可以扩展到模拟其他合成细胞细胞器。
