Lindsay D S, Dubey J P
U.S. Department of Agriculture, Livestock and Poultry Sciences Institute, BARC-East, Beltsville, Maryland 20705.
J Parasitol. 1989 Feb;75(1):163-5.
The development of Neospora caninum isolated from naturally infected dogs was examined in mammalian cell cultures. Tachyzoites developed by endodyogeny when inoculated onto bovine monocyte or bovine cardiopulmonary artery endothelial cell cultures. Tachyzoites were 5.0 by 2.0 microns and had a posteriorly located nucleus. Cytopathogenic effects of parasite development consisted of the formation of holes in the cell monolayer associated with the rupture of infected host cells. Serial passage of tachyzoites was achieved by subinoculation of tachyzoites onto non-infected bovine monocyte cell cultures. It appears that N. caninum can be continuously grown in cell cultures.
对从自然感染犬只分离出的犬新孢子虫的发育情况，在哺乳动物细胞培养物中进行了研究。速殖子接种到牛单核细胞或牛心肺动脉内皮细胞培养物上时，通过内二芽殖法进行发育。速殖子大小为5.0×2.0微米，细胞核位于后端。寄生虫发育产生的细胞病变效应包括在细胞单层中形成孔洞，这与受感染宿主细胞的破裂有关。通过将速殖子再次接种到未感染的牛单核细胞培养物上，实现了速殖子的连续传代。看来犬新孢子虫能够在细胞培养物中持续生长。
