Barta J R, Dubey J P
Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
Parasitol Res. 1992;78(8):689-94. doi: 10.1007/BF00931522.
The antigens recognized by hyperimmune rabbit serum raised against tachyzoites of the NC-1 isolate of Neospora caninum were characterized using chemiluminescent Western blotting, immunogold-silver staining and immunoelectron microscopy. Approximately 20 immunodominant antigens whose relative rates of migration were 16-80 kDa were recognized by the serum in Western blots using reduced or nonreduced parasite antigen preparations. The nonreduced parasite antigens were more strongly recognized by the serum than were the reduced antigen preparations. Immunoelectron microscopy revealed that the rabbit-serum-labeled antigens were localized to some organelles of N. caninum tachyzoites and to the parasitophorous vacuole surrounding them. In particular, antigens found in the dense granules, in the micronemes, and in the posterior portion of the rhoptries were strongly labeled by an indirect immunogold-labeling technique.
利用化学发光免疫印迹法、免疫金银染色法和免疫电子显微镜技术，对用犬新孢子虫NC-1分离株速殖子免疫的兔血清所识别的抗原进行了特性分析。在使用还原或非还原寄生虫抗原制剂的免疫印迹中，血清识别出了大约20种免疫显性抗原，其相对迁移率为16 - 80 kDa。与还原抗原制剂相比，血清对非还原寄生虫抗原的识别更强。免疫电子显微镜显示，兔血清标记的抗原定位于犬新孢子虫速殖子的一些细胞器以及围绕它们的寄生泡。特别是，通过间接免疫金标记技术，在致密颗粒、微线体和棒状体后部发现的抗原被强烈标记。
