Atarbashi Saede, Elahi Maryam, Khani Mehdi, Rakhshan Vahid
Department of Oral and Maxillofacial Pathology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Dent Res J (Isfahan). 2014 Mar;11(2):257-63.
Salivary gland tumors form a major area in the field of oral pathology. B-cell lymphoma -2 (Bcl-2) is an anti-apoptotic gene with up-regulation in various neoplasms. The aim of the present case-control study was to comparatively investigate the expression of Bcl-2 protein in pleomorphic adenoma (PA) and mucoepidermoid carcinoma (MEC) and assess its potential diagnostic role in differentiating these tumors.
A total of 28 cases (18 specimens of PA [control] and 10 blocks of MEC [case]) were selected for analysis by streptavidin-biotin peroxidase method with antibody against Bcl-2. The percentage of positive cells was calculated from a minimum of 1000 neoplastic cells and H-score was identified (% positive cells × intensity of staining). Data were analyzed using two-way ANOVA, t-test, Spearman, Chi-square and Fisher tests (α = 0.05).
bcl-2 expression was shown in 13 cases (71%) of PA and 3 cases (30%) of MEC. In addition, four cases of PA showed strong staining. There was a significant difference between the expression intensity of Bcl-2 in the two tumors (P = 0.048) according to ANOVA. No correlation was observed between Bcl-2 expression with the size and location of tumors (P > 0.05).
Bcl-2 expression might be used for differentiating these tumors. Bcl-2 protein was overexpressed in PA compared with MEC. Hence, it seems that unlike what was observed in PA, Bcl-2 probably does not participate in the pathogenesis of MEC.
涎腺肿瘤是口腔病理学领域的一个主要研究方向。B细胞淋巴瘤-2（Bcl-2）是一种抗凋亡基因，在多种肿瘤中呈上调表达。本病例对照研究的目的是比较研究Bcl-2蛋白在多形性腺瘤（PA）和黏液表皮样癌（MEC）中的表达情况，并评估其在鉴别这些肿瘤中的潜在诊断作用。
选取28例病例（18例PA标本[对照组]和10例MEC组织块[病例组]），采用抗Bcl-2抗体的链霉亲和素-生物素过氧化物酶法进行分析。从至少1000个肿瘤细胞中计算阳性细胞百分比，并确定H评分（阳性细胞百分比×染色强度）。采用双向方差分析、t检验、Spearman检验、卡方检验和Fisher检验对数据进行分析（α = 0.05）。
13例（71%）PA和3例（30%）MEC中显示有Bcl-2表达。此外，4例PA显示强染色。根据方差分析，两种肿瘤中Bcl-2的表达强度存在显著差异（P = 0.048）。未观察到Bcl-2表达与肿瘤大小和位置之间存在相关性（P > 0.05）。
Bcl-2表达可用于鉴别这些肿瘤。与MEC相比，PA中Bcl-2蛋白过度表达。因此，与PA中观察到的情况不同，Bcl-2可能不参与MEC的发病机制。
