Dorado-Morales Pedro, Iglesias Alba, Zafrilla Guillermo, Valero Alejandro, Torres Alejandro, Miravet-Verde Samuel, de Loma Jessica, Mañas Marina, Ruiz Antonio, Corman Alba, Morales Lucas J, Peretó Juli, Vilanova Cristina, Porcar Manuel
Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València , 46980 Paterna, Valencia, Spain.
ACS Synth Biol. 2014 Dec 19;3(12):941-3. doi: 10.1021/sb4001883. Epub 2014 Feb 4.
Bacteria are needed for a vast range of biotechnological processes, which they carry out either as pure cultures or in association with other bacteria and/or fungi. The potential of bacteria as biofactories is hampered, though, by their limited mobility in solid or semisolid media such as agricultural or domestic waste. This work represents an attempt toward overcoming this limitation by associating bacterial biotechnological properties with the transport ability of the nematode Caenorhabditis elegans. We report here biofilm formation on C. elegans by engineered Escherichia coli expressing a Xhenorhabdus nematophila adhesion operon and induction of nematode social feeding behavior (clumping) through an E. coli-mediated iRNA blocking on the expression of FLP-21, a neuropeptide involved in worm solitary behavior.
许多生物技术过程都需要细菌参与，这些过程可以通过纯培养的细菌来完成，也可以通过与其他细菌和/或真菌联合进行。然而，细菌作为生物工厂的潜力受到了限制，因为它们在诸如农业或生活垃圾等固体或半固体介质中的移动性有限。这项研究试图通过将细菌的生物技术特性与秀丽隐杆线虫的运输能力相结合来克服这一限制。我们在此报告，通过表达嗜线虫致病杆菌粘附操纵子的工程化大肠杆菌在秀丽隐杆线虫上形成生物膜，并通过大肠杆菌介导的干扰RNA阻断参与线虫独居行为的神经肽FLP-21的表达，从而诱导线虫的群居进食行为（聚集）。
