水飞蓟素对邻苯二甲酸二（2-乙基己基）酯（DEHP）诱导的肝脏FL83B细胞损伤的保护作用。
Hepatoprotective effect of silymarin on di(2-ethylhexyl)phthalate (DEHP) induced injury in liver FL83B cells.
作者信息
Lo Diana, Wang Yuh-Tai, Wu Ming-Chang
机构信息
Department of Food Science, National Pingtung University of Science and Technology, No. 1, Hsueh Fu Road, Nei-Pu Hsiang, Pingtung 91201, Taiwan.
Life Science Center, Hsing Wu University, No. 101, Sec. 1, Fen-Liao Road, Lin Kou, Taipei 244, Taiwan.
出版信息
Environ Toxicol Pharmacol. 2014 Jul;38(1):112-8. doi: 10.1016/j.etap.2014.05.005. Epub 2014 May 27.
Di(2-ethylhexyl)phthalate (DEHP), is a toxic environmental pollutant in our life which can contaminate air, water, and soil. The hepatoprotective effect of silymarin on DEHP-induced injury in FL83B mouse liver cells was investigated by analyzing the cell viability, lactate dehydrogenase (LDH), alanine aminotransferase (ALT), cell cycle arrest, and cell morphology. The results revealed that cell viability decreased while released LDH and ALT increased with the increase of DEHP concentrations. Moreover, cell population of sub-G1 and S phase increased as the concentrations of DEHP increased. Silymarin at 25 μM achieved the highest hepatoprotective effect and exhibited 79% cell viability while only 46% cell viability was found in DEHP injured control. It was also found to reduce LDH release and cell populations of sub-G1 and S phase. Therefore, silymarin could ameliorate DEHP-induced injury and have potential to be further developed as a natural ingredient of health food against phthalate plasticizers induced liver injury.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是我们生活中的一种有毒环境污染物，可污染空气、水和土壤。通过分析细胞活力、乳酸脱氢酶（LDH）、丙氨酸转氨酶（ALT）、细胞周期阻滞和细胞形态，研究了水飞蓟素对DEHP诱导的FL83B小鼠肝细胞损伤的保护作用。结果显示，随着DEHP浓度的增加，细胞活力下降，而释放的LDH和ALT增加。此外，随着DEHP浓度的增加，亚G1期和S期的细胞群体增加。25μM的水飞蓟素具有最高的肝保护作用，细胞活力达到79%，而在DEHP损伤的对照组中细胞活力仅为46%。还发现它能减少LDH释放以及亚G1期和S期的细胞群体。因此，水飞蓟素可以改善DEHP诱导的损伤，并有潜力作为一种天然成分进一步开发为保健食品，用于对抗邻苯二甲酸酯类增塑剂诱导的肝损伤。
Zotero 插件