Faculty of Dental Medicine, University of Porto, Portugal Nephrology Research and Development Unit, University of Porto, Portugal.
Institute of Molecular Pathology and Immunology, University of Porto, Portugal Faculty of Sciences, University of Porto, Portugal
Med Mycol. 2014 Jul;52(5):498-505. doi: 10.1093/mmy/myu027. Epub 2014 Jun 15.
Oral microbiota is one of the most complex and diverse microbial communities in the human body. In the present study, we aimed to characterize oral fungi biodiversity and stability over time in a group of healthy participants with good oral health. Oral health and oral fungal microbiota were evaluated in 40 healthy individuals. A follow-up of 10 participants was carried out 28 weeks and 30 weeks after the first sampling. Oral rinse was collected and incubated in a fungal selective medium at 25ºC and 37ºC for 7 days. Fungi were identified based on macro- and microscopic morphology. API/ID32C was used for yeast identification, and molecular techniques were used to identify the most prevalent nonidentified moulds, mainly by sequencing 18S and internally transcribed spacer regions. Moulds were recovered from all participants and yeast from 92.5%. The most frequently isolated fungi were Candida spp., Rhodotorula spp., Penicillium spp., Aspergillus spp., and Cladosporium spp. The oral fungal community presented a high interindividual variability, but the frequency and quantification of each fungal taxon was constant over the 30-week observation period, showing a consistent intraindividual stability over time. The intraindividual stability opposed to interindividual variability may suggest a common and a variable group of fungi in the oral cavity.
口腔微生物群是人体中最复杂和最多样化的微生物群落之一。在本研究中，我们旨在描述一组口腔健康良好的健康参与者的口腔真菌生物多样性和随时间的稳定性。对 40 名健康个体的口腔健康和口腔真菌微生物群进行了评估。在第一次采样后 28 周和 30 周对 10 名参与者进行了随访。采集口腔冲洗液并在真菌选择性培养基中于 25°C 和 37°C 孵育 7 天。根据宏观和微观形态学鉴定真菌。使用 API/ID32C 鉴定酵母，并且使用分子技术鉴定最常见的未鉴定霉菌，主要通过测序 18S 和内部转录间隔区。从所有参与者中均可回收霉菌，从 92.5%的参与者中可回收酵母。最常分离的真菌为 Candida spp.、Rhodotorula spp.、Penicillium spp.、Aspergillus spp. 和 Cladosporium spp.。口腔真菌群落个体间变异性很大，但每个真菌分类群的频率和定量在 30 周的观察期内保持不变，表现出随时间的个体内稳定性。个体内的稳定性与个体间的变异性相反，可能表明口腔中有一组共同且可变的真菌。
