Sahoo Naresh Kumar, Pakshirajan Kannan, Ghosh Pranab Kumar
Department of Chemistry, Environmental Science and Technology Section, Institute of Technical Education and Research, Siksha'O'Anusandhan University, Bhubaneswar, 751030, Orissa, India,
Biodegradation. 2014 Sep;25(5):705-18. doi: 10.1007/s10532-014-9693-2. Epub 2014 Jun 17.
Bromophenol is listed as priority pollutant by U.S. EPA, however, there is no report so far on its removal in mixed pollutants system by any biological reactor operated in continuous mode. Furthermore, bromophenol along with chlorophenol and nitrophenol are usually the major constituents of paper pulp and pesticide industrial effluent. The present study investigated simultaneous biodegradation of these three pollutants with specially emphasis on substrate competition and crossed inhibition by Arthrobacter chlorophenolicus A6 in an upflow packed bed reactor (UPBR). A 2(3) full factorial design was employed with these pollutants at two different levels by varying their influent concentration in the range of 250-450 mg l(-1). Almost complete removal of all these pollutants and 97 % effluent toxicity removal were achieved in the UPBR at a pollutant loading rate of 1707 mg l(-1) day(-1) or lesser. However, at higher loading rates, the reactor performance deteriorated due to transient accumulation of toxic intermediates. Statistical analysis of the results revealed a strong negative interaction of 4-CP on 4-NP biodegradation. On the other hand, interaction effect between 4-CP and 4-BP was found to be insignificant. Among these three pollutants 4-NP preferentially degraded, however, 4-CP exerted more inhibitory effect on 4-NP biodegradation. This study demonstrated the potential of A. chlorophenolicus A6 for biodegradation of 4-BP in mixed pollutants system by a flow through UPBR system.
溴酚被美国环境保护局列为优先污染物，然而，目前尚无关于任何以连续模式运行的生物反应器在混合污染物系统中去除溴酚的报道。此外，溴酚与氯酚和硝基酚通常是纸浆和农药工业废水的主要成分。本研究以 2(3) 全因子设计，通过将这三种污染物的进水浓度在 250 - 450 mg l(-1) 范围内变化，以两种不同水平研究了嗜氯节杆菌 A6 在升流式填充床反应器 (UPBR) 中对这三种污染物的同步生物降解，特别强调了底物竞争和交叉抑制。在污染物负荷率为 1707 mg l(-1) 天(-1) 或更低时，在 UPBR 中几乎实现了所有这些污染物的完全去除以及 97% 的出水毒性去除。然而，在较高负荷率下，由于有毒中间产物的瞬时积累，反应器性能恶化。结果的统计分析表明 4 - CP 对 4 - NP 生物降解有强烈的负相互作用。另一方面，发现 4 - CP 和 4 - BP 之间的相互作用效应不显著。在这三种污染物中，4 - NP 优先降解，然而，4 - CP 对 4 - NP 生物降解的抑制作用更大。本研究证明了嗜氯节杆菌 A6 通过连续流 UPBR 系统在混合污染物系统中对 4 - BP 进行生物降解的潜力。
