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利用新型上流式填充床反应器中的节杆菌 A6 对对硝基苯酚进行生物降解。

Biodegradation of p-nitrophenol using Arthrobacter chlorophenolicus A6 in a novel upflow packed bed reactor.

机构信息

Center for Environment, Indian Institute of Technology Guwahati, 781039, India.

出版信息

J Hazard Mater. 2011 Jun 15;190(1-3):729-37. doi: 10.1016/j.jhazmat.2011.03.106. Epub 2011 Apr 4.

DOI:10.1016/j.jhazmat.2011.03.106
PMID:21501928
Abstract

A novel packed bed reactor (PBR) was designed with cross flow aeration at multiple ports along the depth to improve the hydrodynamic conditions of the reactor, and the biodegradation efficiency of Arthrobacter chlorophenolicus A6 on p-nitrophenol (PNP) removal in PBR at different PNP loading rates were evaluated. The novel PBR was designed to improve the hydrodynamic features such as mixing time profile (t(m95)), oxygen mass transfer coefficient (k(L)a), and overall gas hold up capacity (ɛ(G)) of the reactor. PNP concentration in the influent was varied between 600 and 1400 mg l(-1) whereas the hydraulic retention time (HRT) in the reactor was varied between 18 and 7.5h. Complete removal of PNP was achieved in the reactor up to a PNP loading rate of 2787 mg l(-1)d(-1). More than 99.9% removal of PNP was achieved in the reactor for an influent concentration of 1400 mg l(-1) and at 18 h HRT. In the present study, PNP was utilized as sole source of carbon and energy by A. chlorophenolicus A6. Furthermore, the bioreactor showed good compatibility in handling shock loading of PNP.

摘要

设计了一种新型填充床反应器(PBR),在深度方向上沿多个端口采用交叉流动曝气,以改善反应器的流体动力学条件,并评估了 Arthrobacter chlorophenolicus A6 在不同 p-硝基苯酚(PNP)负荷率下在 PBR 中对 PNP 去除的生物降解效率。新型 PBR 的设计旨在改善反应器的流体动力学特性,如混合时间分布(t(m95))、氧传质系数(k(L)a)和总体气体持液量(ɛ(G))。进水 PNP 浓度在 600 至 1400 mg l(-1)之间变化,而反应器中的水力停留时间(HRT)在 18 至 7.5 h 之间变化。在反应器中,PNP 的负荷率高达 2787 mg l(-1)d(-1)时,可实现 PNP 的完全去除。在反应器中,当进水浓度为 1400 mg l(-1)且 HRT 为 18 h 时,可实现 PNP 的去除率超过 99.9%。在本研究中,PNP 被 Arthrobacter chlorophenolicus A6 用作唯一的碳源和能源。此外,该生物反应器在处理 PNP 的冲击负荷方面表现出良好的兼容性。

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