Rushatamukayanunt Pradit, Morita Kei-ichi, Matsukawa Sho, Harada Hiroyuki, Shimamoto Hiroaki, Tomioka Hirofumi, Omura Ken
Oral and Maxillofacial Surgery, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan E-mail :
Asian Pac J Cancer Prev. 2014;15(10):4135-41. doi: 10.7314/apjcp.2014.15.10.4135.
Human papillomaviruses (HPV) may play an important role as one of the possible etiologies of oral squamous cell carcinoma (OSCC). The present study aimed to investigate the association between HPV and OSCC in young Japanese patients by examining the presence of HPV DNA and surrogate markers in OSCC tissues.
Forty young patients with OSCC whose surgical specimens were available were analyzed and compared with 40 patients randomly recruited from a pool of patients aged >40 years. HPV DNA was detected using the polymerase chain reaction-based AMPLICOR(®) HPV test, and surrogate markers of HPV infection were analyzed using immunohistochemical techniques to detect p16(INK4a) and p53.
Only two (5%) young patients and one (2.5%) older patient were positive for HPV DNA. p16(INK4a) overexpression was identified in six (15%) young patients. p53 staining levels were not high in tissues of most young patients (27 patients, 67.5%). HPV DNA status did not significantly correlate with p16(INK4a) expression levels. Profiles of increased levels of p16(INK4a) expression with diminished levels of p53 staining were not associated with the presence of HPV DNA. The combined p53 with p16(INK4a) profiles were significantly correlated with alcohol consumption in younger patients (p=0.006).
RESULTS of the present study indicate that HPV is less likely to cause OSCC in young Japanese patients, and the p16(INK4a) expression level is not an appropriate surrogate marker for HPV infection in OSCC.
人乳头瘤病毒（HPV）可能作为口腔鳞状细胞癌（OSCC）潜在病因之一发挥重要作用。本研究旨在通过检测OSCC组织中HPV DNA和替代标志物的存在情况，调查年轻日本患者中HPV与OSCC之间的关联。
分析40例有手术标本的年轻OSCC患者，并与从40岁以上患者群体中随机招募的40例患者进行比较。使用基于聚合酶链反应的AMPLICOR（®）HPV检测法检测HPV DNA，并使用免疫组织化学技术分析HPV感染的替代标志物以检测p16（INK4a）和p53。
仅2例（5%）年轻患者和1例（2.5%）老年患者HPV DNA呈阳性。6例（15%）年轻患者中发现p16（INK4a）过表达。大多数年轻患者（27例，67.5%）的组织中p53染色水平不高。HPV DNA状态与p16（INK4a）表达水平无显著相关性。p16（INK4a）表达水平升高而p53染色水平降低的情况与HPV DNA的存在无关。年轻患者中p53与p16（INK4a）联合情况与饮酒显著相关（p = 0.006）。
本研究结果表明，HPV在年轻日本患者中引起OSCC的可能性较小，且p16（INK4a）表达水平不是OSCC中HPV感染的合适替代标志物。
