Department of Head and Neck Oncology and Surgery, NKI/Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands.
Int J Cancer. 2012 Apr 15;130(8):1806-12. doi: 10.1002/ijc.26195. Epub 2011 Aug 2.
The role of human papillomavirus (HPV) in head and neck squamous cell carcinoma (HNSCC) development has been recognized only in the last decade. Although younger patients develop HNSCC associated with HPV, the incidence in young patients has not been studied. Forty-five young HNSCC patients (<40 years) were tested for HPV and the expression of p16(ink4a) and p53 in tumor biopsies. The presence of HPV was correlated with the absence and presence of alcohol and tobacco exposure. Paraffin-embedded, archival biopsy materials from HNSCC of 45 patients younger than 40 years were analyzed. HPV subtypes were identified by PCR followed by genotyping. Expression of p16(ink4a) and p53 were determined by immunohistochemistry. Fourteen (31%) of the HNSCC specimens from 45 patients unequivocally exhibited HPV16 positivity. Sixty percentage of the oropharyngeal tumors and 5% of the oral cavity tumors were HPV16 positive. P16(ink4a) overexpression was detected in 93% of the HPV16-positive tumors. None of the HPV16 tumors showed p53 overexpression. There was no association of HPV positivity with (lack of) exposure to alcohol and smoking. HPV association was not exclusively detected in nonsmoking, nondrinking young HNSCC patients. The presence of p16(ink4a) accumulation and the absence of p53 overexpression are good surrogate markers for HPV-associated HNSCC.
人乳头瘤病毒（HPV）在头颈部鳞状细胞癌（HNSCC）发展中的作用仅在过去十年中得到认识。尽管年轻患者会发展与 HPV 相关的 HNSCC，但年轻患者的发病率尚未得到研究。45 名年轻的 HNSCC 患者（<40 岁）接受了 HPV 检测以及肿瘤活检中 p16(ink4a)和 p53 的表达检测。HPV 的存在与酒精和烟草暴露的有无相关。对 45 名年龄小于 40 岁的 HNSCC 患者的存档石蜡包埋活检材料进行了分析。通过 PCR 后进行基因分型鉴定 HPV 亚型。通过免疫组织化学检测 p16(ink4a)和 p53 的表达。在 45 名患者的 14 份（31%）HNSCC 标本中，HPV16 阳性明确。60%的口咽肿瘤和 5%的口腔肿瘤为 HPV16 阳性。93%的 HPV16 阳性肿瘤中检测到 p16(ink4a)过表达。没有 HPV16 肿瘤显示 p53 过表达。HPV 阳性与（缺乏）暴露于酒精和吸烟无关。HPV 关联并非仅在不吸烟、不饮酒的年轻 HNSCC 患者中检测到。p16(ink4a)积聚的存在和 p53 过表达的缺失是 HPV 相关 HNSCC 的良好替代标志物。
